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学科主题: 基础医学
题名:
Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert
作者: Lau, LT; Collins, RA; Yiu, SH; Xing, J; Yu, ACH
关键词: roundup ready ; rearrangement ; GM
刊名: FOOD CONTROL
发表日期: 2004-09-01
DOI: 10.1016/j.foodcont.2003.03.001
卷: 15, 期:6, 页:471-478
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Food Science & Technology
研究领域[WOS]: Food Science & Technology
关键词[WOS]: TRANSPOSABLE GENETIC ELEMENTS ; REARRANGEMENTS
英文摘要:

The genetically modified (GM) Roundup Ready(R) soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure of the DNA surrounding the insertion has not been fully characterized. A semi-nested PCR method identified the rearranged soybean DNA in samples of raw, partially processed and highly processed food at a level of 0.01%. The 534 bp rearranged segment contained a contiguous portion at least 238 bp long that was also identified in non-GM soybeans. Specific combinations of semi-nested PCR primers differentiated GM and non-GM soybean DNA. These studies confirm that the rearranged DNA originates from the soybean genome and does not involve the introduction of non-soybean genetic material. (C) 2003 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000222056300009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54077
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Hong Kong DNA Chips Ltd, Cosmos Ctr, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100871, Peoples R China

Recommended Citation:
Lau, LT,Collins, RA,Yiu, SH,et al. Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert[J]. FOOD CONTROL,2004,15(6):471-478.
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