IR@PKUHSC  > 北京大学基础医学院  > 神经生物学系
学科主题基础医学
Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert
Lau, LT; Collins, RA; Yiu, SH; Xing, J; Yu, ACH
关键词roundup ready rearrangement GM
刊名FOOD CONTROL
2004-09-01
DOI10.1016/j.foodcont.2003.03.001
15期:6页:471-478
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Food Science & Technology
研究领域[WOS]Food Science & Technology
关键词[WOS]TRANSPOSABLE GENETIC ELEMENTS ; REARRANGEMENTS
英文摘要

The genetically modified (GM) Roundup Ready(R) soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure of the DNA surrounding the insertion has not been fully characterized. A semi-nested PCR method identified the rearranged soybean DNA in samples of raw, partially processed and highly processed food at a level of 0.01%. The 534 bp rearranged segment contained a contiguous portion at least 238 bp long that was also identified in non-GM soybeans. Specific combinations of semi-nested PCR primers differentiated GM and non-GM soybean DNA. These studies confirm that the rearranged DNA originates from the soybean genome and does not involve the introduction of non-soybean genetic material. (C) 2003 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000222056300009
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54077
专题北京大学基础医学院_神经生物学系
作者单位1.Hong Kong DNA Chips Ltd, Cosmos Ctr, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lau, LT,Collins, RA,Yiu, SH,et al. Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert[J]. FOOD CONTROL,2004,15(6):471-478.
APA Lau, LT,Collins, RA,Yiu, SH,Xing, J,&Yu, ACH.(2004).Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert.FOOD CONTROL,15(6),471-478.
MLA Lau, LT,et al."Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert".FOOD CONTROL 15.6(2004):471-478.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Detection and charac(384KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lau, LT]的文章
[Collins, RA]的文章
[Yiu, SH]的文章
百度学术
百度学术中相似的文章
[Lau, LT]的文章
[Collins, RA]的文章
[Yiu, SH]的文章
必应学术
必应学术中相似的文章
[Lau, LT]的文章
[Collins, RA]的文章
[Yiu, SH]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。