|Role of paxillin in colorectal carcinoma and its relationship to clinicopathological features|
|Yin Hongfang1; Zhang Quanwen2; Wang Xin2; Li Ting1; Wan Yuanlian2; Liu Yucun2; Zhu Jing3|
|关键词||Cell Cycle Apoptosis Colorectal Neoplasms Paxillin Clinicopathology|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||FOCAL ADHESION KINASE ; CELL LUNG-CANCER ; PROTEIN PAXILLIN ; EXPRESSION ; APOPTOSIS ; MIGRATION ; INVASION ; BCL-2 ; TRANSFORMATION ; P210(BCR/ABL)|
Background Colorectal carcinoma is one of the most common malignant tumors. Despite advances in therapy, mortality is still very high. The aim of this study was to evaluate the expression of paxillin in the human colon adenocarcinoma cell line SW480 and its role in cell cycle and apoptosis. We also investigated the expression of paxillin in colorectal carcinoma tissues and its relationship to clinicopathological features and survival.
Methods Paxillin short hairpin RNA (shRNA) was constructed and transfected into the colon adenocarcinoma cell line SW480. The influence of paxillin shRNA on the cell cycle and cell apoptosis was analyzed by flow cytometry. Immunohistochemistry staining was used to assess the expression of paxillin and its association with the expression of carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 19-9, p53 and Bcl-2 in 102 patients with primary colorectal carcinoma. Western blotting was also used to investigate the expression of paxillin. Medical records were reviewed and a clinicopathological analysis was performed.
Results In vitro, the percentage of cells in S phase was (45.23 +/- 1.05)%, (43.53 +/- 1.23)%, and (36.13 +/- 0.57)% in the blank control group, negative control group, and paxillin shRNA group respectively. It was significantly decreased in the paxillin shRNA group (P=0.000). The early apoptosis index of the paxillin shRNA group (17.2 +/- 1.18%) was significantly increased compared to the control shRNA group ((13.17 +/- 1.15)%, P=0.013). Paxillin was positive in 71 (69.6%) patients, and it was found to be overexpressed in tumor tissues compared with normal adjacent tissues. Paxillin positive rate was higher in patients who are less than 50-years old (100.0% vs. 65.6%, P=0.016). Paxillin expression was associated with a high histologic grade of carcinoma (81.4% vs. 61.0%, P=0.031), a high rate of regional lymph node metastasis (22.5% vs. 13.0%, P=0.031), mesenteric artery lymph node metastasis (100.0% vs. 64.8%, P=0.008), distant metastasis (94.1% vs. 64.7%, P=0.016) and a high Tumor Node Metastasis (TNM) stage (94.1%, 73.2%, 60.0%, and 50%, P=0.030). Multivariate analyses revealed that recurrence was associated with the rate of regional lymph node metastasis (P=0.001) and paxillin expression (P=0.024). Multivariate analysis indicated that the overall survival is related to the TNM stage (P=0.000).
Conclusions In vitro, paxillin may promote cell proliferation and inhibit apoptosis in SW480 cells. Paxillin may be a potential metastasis predictor, and an independent prognosis factor of recurrence. It may also be related to poor patient outcomes, but was not an independent predictor of survival.
|作者单位||1.Peking Univ, Hosp 1, Surg Lab, Beijing 100034, Peoples R China|
2.Peking Univ, Hosp 1, Dept Pathol, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China
|Yin Hongfang,Zhang Quanwen,Wang Xin,et al. Role of paxillin in colorectal carcinoma and its relationship to clinicopathological features[J]. CHINESE MEDICAL JOURNAL,2014,127(3):423-429.|
|APA||Yin Hongfang.,Zhang Quanwen.,Wang Xin.,Li Ting.,Wan Yuanlian.,...&Zhu Jing.(2014).Role of paxillin in colorectal carcinoma and its relationship to clinicopathological features.CHINESE MEDICAL JOURNAL,127(3),423-429.|
|MLA||Yin Hongfang,et al."Role of paxillin in colorectal carcinoma and its relationship to clinicopathological features".CHINESE MEDICAL JOURNAL 127.3(2014):423-429.|