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学科主题: 基础医学
题名:
The comet assay: A sensitive method for detecting DNA damage in individual cells
作者: Liao, Wenjuan1; McNutt, Michael A.2; Zhu, Wei-Guo1
关键词: Comet assay ; Single cell gel ; DNA strand breaks ; DNA damage and repair
刊名: METHODS
发表日期: 2009-05-01
DOI: 10.1016/j.ymeth.2009.02.016
卷: 48, 期:1, 页:46-53
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: GEL-ELECTROPHORESIS ASSAY ; HUMAN-LYMPHOCYTES ; IN-VITRO ; APOPTOSIS ; REPAIR ; 5-AZA-2&prime ; -DEOXYCYTIDINE ; PATHWAY ; P53/P21(WAF1/CIP1) ; METHYLTRANSFERASE ; PROLIFERATION
英文摘要:

The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells, and the year 2009 represents the 25th anniversary of the first description of this methodology in 1984. Over time this method has been improved, but is still not completely standardized, and variations are currently in widespread use with emphasis on applications in research and genetic toxicology. Here we review the principles of the comet assay and cite key studies that have focused on this assay in the past 25 years. In addition, we present an example of how the comet assay was used in our laboratory for studying the induction of DNA damage in human lung cancer cells after differing doses of the cytosine analog 5-aza-2′-deoxycytidine (5-aza-CdR). Finally, some insights into the potential of this assay in cancer research and work in related fields are offered. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30425017 ; 30670417 ; 30621002 ; 2005CB522403 ; 2006CB910300
WOS记录号: WOS:000267143800008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54108
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Key Lab Carcinogenesis & Translat Res, Minist Educ,Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China

Recommended Citation:
Liao, Wenjuan,McNutt, Michael A.,Zhu, Wei-Guo. The comet assay: A sensitive method for detecting DNA damage in individual cells[J]. METHODS,2009,48(1):46-53.
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