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学科主题基础医学
The comet assay: A sensitive method for detecting DNA damage in individual cells
Liao, Wenjuan1; McNutt, Michael A.2; Zhu, Wei-Guo1
关键词Comet assay Single cell gel DNA strand breaks DNA damage and repair
刊名METHODS
2009-05-01
DOI10.1016/j.ymeth.2009.02.016
48期:1页:46-53
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]GEL-ELECTROPHORESIS ASSAY ; HUMAN-LYMPHOCYTES ; IN-VITRO ; APOPTOSIS ; REPAIR ; 5-AZA-2&prime ; -DEOXYCYTIDINE ; PATHWAY ; P53/P21(WAF1/CIP1) ; METHYLTRANSFERASE ; PROLIFERATION
英文摘要

The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells, and the year 2009 represents the 25th anniversary of the first description of this methodology in 1984. Over time this method has been improved, but is still not completely standardized, and variations are currently in widespread use with emphasis on applications in research and genetic toxicology. Here we review the principles of the comet assay and cite key studies that have focused on this assay in the past 25 years. In addition, we present an example of how the comet assay was used in our laboratory for studying the induction of DNA damage in human lung cancer cells after differing doses of the cytosine analog 5-aza-2′-deoxycytidine (5-aza-CdR). Finally, some insights into the potential of this assay in cancer research and work in related fields are offered. (C) 2009 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000267143800008
项目编号30425017 ; 30670417 ; 30621002 ; 2005CB522403 ; 2006CB910300
引用统计
被引频次:137[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54108
专题北京大学基础医学院_病理学系
北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Key Lab Carcinogenesis & Translat Res, Minist Educ,Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
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GB/T 7714
Liao, Wenjuan,McNutt, Michael A.,Zhu, Wei-Guo. The comet assay: A sensitive method for detecting DNA damage in individual cells[J]. METHODS,2009,48(1):46-53.
APA Liao, Wenjuan,McNutt, Michael A.,&Zhu, Wei-Guo.(2009).The comet assay: A sensitive method for detecting DNA damage in individual cells.METHODS,48(1),46-53.
MLA Liao, Wenjuan,et al."The comet assay: A sensitive method for detecting DNA damage in individual cells".METHODS 48.1(2009):46-53.
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