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Effects of WNT10A on Proliferation and Differentiation of Human Dental Pulp Cells
Zhang, Zhichun1; Guo, Qingru1; Tian, Hua1; Lv, Ping1; Zhou, Chunyan2; Gao, Xuejun1
关键词Dentin-pulp Regeneration Dentin Repair Human Dental Pulp Cells Wnt10a
刊名JOURNAL OF ENDODONTICS
2014-10-01
DOI10.1016/j.joen.2014.07.009
40期:10页:1593-1599
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China (NSFC) ; 111 Project of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China (NSFC) ; 111 Project of China ; Specialized Research Fund for the Doctoral Program of Higher Education
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]STEM-CELLS ; TOOTH MORPHOGENESIS ; ODONTOGENIC DIFFERENTIATION ; ODONTOBLAST DIFFERENTIATION ; PROMOTES PROLIFERATION ; EXPRESSION ; OSTEOBLAST ; MINERALIZATION ; PATHWAY ; GROWTH
英文摘要

Introduction: Wingless-type MMTV integration site family, member 10A (WNT10A) plays crucial roles in odontogenesis. The aim of this study was to investigate the effects of WNT10A on human dental pulp cells (DPCs), which contain a mixed population of cells, including stem and progenitor cells, and participate in dentin repair or dentin-pulp regeneration. Methods: Healthy human premolars extracted for orthodontic reasons were used as a study model. The expression of WNT10A protein in dental pulp was determined by immunohistochemistry. The messenger RNA expression of WNT10A and Wnt-related genes was analyzed by semiquantitative reverse-transcription polymerase chain reaction. DPCs were enzymatically separated from pulp tissues, cultured, and passaged. The biological effects of WNT10A on DPCs were investigated using recombinant lentivirus encoding WNT10A complementary DNA. WNT10A-induced changes in DPC proliferation were assessed by methyltetrazolium assay and flow cytometry. In order to determine the effects of WNT10A on DPC differentiation, the activity of alkaline phosphatase (ALP), an early marker of odontoblastic differentiation, was assessed using an ALP activity assay kit, and the expression levels of odontoblast-specific genes, including DSPP, DMP1, ALP, and COL1A1, were detected by quantitative polynnerase chain reaction and Western blot. Results: WNT10A protein was clearly identified in the cytoplasm of DPCs. Semiquantitative reverse-transcription polymerase chain reaction indicated the expression of WNT10A and Wnt-related genes in pulp tissues as well as in passaging DPCs. Lentiviral overexpression of WNT10A enhanced proliferation of DPCs and down-regulated ALP, activity and the expression of odontoblast-specific genes. Conclusions: WNT10A promotes the proliferation of DPCs and negatively regulates their odontoblastic differentiation.

语种英语
所属项目编号81070800 ; 81300839 ; B07001 ; 20110001130001 ; 20130001120109
资助者National Natural Science Foundation of China (NSFC) ; 111 Project of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China (NSFC) ; 111 Project of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号WOS:000342888400013
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54116
专题基础医学院
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Zhichun,Guo, Qingru,Tian, Hua,et al. Effects of WNT10A on Proliferation and Differentiation of Human Dental Pulp Cells[J]. JOURNAL OF ENDODONTICS,2014,40(10):1593-1599.
APA Zhang, Zhichun,Guo, Qingru,Tian, Hua,Lv, Ping,Zhou, Chunyan,&Gao, Xuejun.(2014).Effects of WNT10A on Proliferation and Differentiation of Human Dental Pulp Cells.JOURNAL OF ENDODONTICS,40(10),1593-1599.
MLA Zhang, Zhichun,et al."Effects of WNT10A on Proliferation and Differentiation of Human Dental Pulp Cells".JOURNAL OF ENDODONTICS 40.10(2014):1593-1599.
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