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学科主题: 临床医学
题名:
INNO-LiPA HBV genotyping is highly consistent with direct sequencing and sensitive in detecting B/C mixed genotype infection in Chinese chronic hepatitis B patients and asymptomatic HBV carriers
作者: Yang, Ruifeng2; Cong, Xu2; Xu, Zhihui1; Xu, Dongping1; Huang, Weijin3; Maertens, Rikkert4; Wei, Lai2
关键词: Hepatitis B virus ; Genotype ; Line probe assay
刊名: CLINICA CHIMICA ACTA
发表日期: 2010-12-14
DOI: 10.1016/j.cca.2010.08.011
卷: 411, 期:23-24, 页:1951-1956
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: SURFACE-ANTIGEN ; VIRUS GENOTYPE ; THERAPY ; ASSAY
英文摘要:

Background: Different HBV genotypes have effect on the progression and outcome of chronic hepatitis B (CHB) and on the response to antiviral therapy. Accurate and sensitive genotyping methods are needed in clinical practice.

Methods: In this multicenter retrospective study, 504 HBV DNA positive serum or plasma samples of CHB patients or asymptomatic carriers were genotyped by INNO-LiPA HBV genotyping and direct sequencing. A part of the consistent and discrepant results were confirmed by clonal sequencing.

Results: Except two samples with indeterminate results by INNO-LiPA, 463 had the same genotype results by INNO-LiPA and direct sequencing with a concordance of 92.23% (463/502). However 24 had completely inconsistent results by the two methods, seven of which were confirmed by clonal sequencing as the same genotype as by direct sequencing. Fifteen samples were determined as B/C mixed genotypes by INNO-LiPA but singe B or C genotype by direct sequencing, five of which were confirmed by clonal sequencing as B/C mixed infection. Further studies showed INNO-LiPA could detect 6% of genotype B and genotype C among mixed genotype samples at HBV DNA >= 3x10(3) IU/ml.

Conclusions: INNO-LiPA is accurate and sensitive in detecting B, C. D and B/C mixed genotype infection in Chinese CHB patients and asymptomatic carriers. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2005CB522902 ; 2007CB512900 ; 2006AA02A410 ; 30571639 ; 2008ZX10002-012 ; 2008ZX10002-013
项目资助者: Chinese Basic Research Foundation ; 863 program ; NSFC ; National Science and Technology Key Project ; Ministry of Health
WOS记录号: WOS:000283916600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54121
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.302 Hosp PLA, Viral Hepatitis Res Lab, Inst Infect Dis, Beijing 100039, Peoples R China
2.Peking Univ, Inst Hepatol, Peking Univ Peoples Hosp, Beijing 100044, Peoples R China
3.Natl Inst Control Pharmaceut & Biol Prod, Beijing 100050, Peoples R China
4.Innogenetics NV, Hepatitis Diagnost Program, B-9052 Ghent, Belgium

Recommended Citation:
Yang, Ruifeng,Cong, Xu,Xu, Zhihui,et al. INNO-LiPA HBV genotyping is highly consistent with direct sequencing and sensitive in detecting B/C mixed genotype infection in Chinese chronic hepatitis B patients and asymptomatic HBV carriers[J]. CLINICA CHIMICA ACTA,2010,411(23-24):1951-1956.
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