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学科主题: 药学
题名:
Identification and Characterization of Three Novel Nuclear Export Signals in the Influenza A Virus Nucleoprotein
作者: Yu, Maorong1,2; Liu, Xiaoling1; Cao, Shuai1,3; Zhao, Zhendong1,3; Zhang, Ke1,3; Xie, Qing1; Chen, Caiwei1,3; Gao, Shengyan1,3; Bi, Yuhai1; Sun, Lei1; Ye, Xin1; Gao, George F.1; Liu, Wenjun1,3,4
刊名: JOURNAL OF VIROLOGY
发表日期: 2012-05-01
DOI: 10.1128/JVI.06159-11
卷: 86, 期:9, 页:4970-4980
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: MESSENGER-RNAS ; RIBONUCLEOPROTEIN COMPLEXES ; LOCALIZATION SEQUENCES ; MUTATIONAL ANALYSIS ; PROTEIN ; REPLICATION ; BINDING ; IMPORT ; ALPHA ; CELLS
英文摘要:

The nuclear export of the influenza A virus ribonucleoprotein (vRNP) is crucial for virus replication. As a major component of the vRNP, nucleoprotein (NP) alone can also be shuttled out of the nucleus by interacting with chromosome region maintenance 1 (CRM1) and is therefore hypothesized to promote the nuclear export of the vRNP. In the present study, three novel nuclear export signals (NESs) of the NP-NES1, NES2, and NES3-were identified as being responsible for mediating its nuclear export. The nuclear export of NES3 was CRM1 dependent, whereas that of NES1 or NES2 was CRM1 independent. Inactivation of these NESs led to an overall nuclear accumulation of NP. Mutation of all three NP-NESs significantly impaired viral replication. Based on structures of influenza virus NP oligomers, these three hydrophobic NESs are found present on the surface of oligomeric NPs. Functional studies indicated that oligomerization is also required for nuclear export of NP. Together, these results suggest that the nuclear export of NP is important for virus replication and relies on its NESs and oligomerization.

语种: 英语
所属项目编号: 973 ; 2011CB504705 ; KSCX2-YW-R-158 ; 30972185 ; 30901073 ; 2010BAD04B01 ; 6102018 ; 81021003
项目资助者: National Basic Research Program of China ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Key Technologies Research and Development Program of China ; Beijing Municipal Natural Science Foundation ; Innovative Research Group of the National Natural Science Foundation of China (NSFC)
WOS记录号: WOS:000302777800022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54124
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing, Peoples R China

Recommended Citation:
Yu, Maorong,Liu, Xiaoling,Cao, Shuai,et al. Identification and Characterization of Three Novel Nuclear Export Signals in the Influenza A Virus Nucleoprotein[J]. JOURNAL OF VIROLOGY,2012,86(9):4970-4980.
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