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学科主题: 口腔医学
题名:
Hydrogen sulfide accelerates cell cycle progression in oral squamous cell carcinoma cell lines
作者: Ma, Z.1; Bi, Q.2; Wang, Y.3
关键词: hydrogen sulfide ; squamous cell carcinoma ; cell cycle ; proliferation
刊名: ORAL DISEASES
发表日期: 2015-03-01
DOI: 10.1111/odi.12223
卷: 21, 期:2, 页:156-162
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: COLON-CANCER CELLS ; DNA-DAMAGE ; INDUCED APOPTOSIS ; EPITHELIAL-CELLS ; NUCLEAR ANTIGEN ; PROTEIN ; PHOSPHORYLATION ; HALITOSIS ; SUBUNIT ; ROLES
英文摘要:

OBJECTIVE: To investigate the cell cycle regulator role of the third gaseous transmitter hydrogen sulfide (H2S) in three oral SCC cell lines by using NaHS, a donor of H2S.

METHODS: The synchronized oral squamous cell carcinoma cell lines (Cal27, GNM, and WSU-HN6) were treated with different concentrations of NaHS and then subjected to cell proliferation, cell cycle, and Western blot analyses.

RESULTS: The CCK-8 assay results showed that the exogenously administered H2S donor, NaHS, induced CAL-27, and GNM cell proliferation in a concentration-dependent manner, and the cell cycle analysis indicated that NaHS accelerated cell cycle progression of the synchronized CAL-27, GNM, and WSU-HN6 cells. Western blot analysis revealed that the cell cycle regulatory genes RPA70 and RB1 were significantly down-regulated and that proliferating cell nuclear antigen (PCNA) and CDK4 were markedly up-regulated by NaHS at specific time points in the cell cycle. In addition, our results indicated that the phosphorylation of Akt and Erk1/2 was involved in exogenous H2S-induced oral SCC cell proliferation.

CONCLUSIONS: H2S is a potential pro-proliferative factor of human oral SCC cells that accelerates the progression of the SCC cell cycle; thus, H2S plays a deleterious role in oral SCC cancer development.

语种: 英语
所属项目编号: 81271150 ; 1019
项目资助者: National Nature Science Foundation of China ; Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Program Foundation
WOS记录号: WOS:000349986500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54127
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Special Dent, Beijing 100081, Peoples R China
2.Hosp Oral Dis Prevent & Treatment, Dept Oral Surg, Harbin, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Ma, Z.,Bi, Q.,Wang, Y.. Hydrogen sulfide accelerates cell cycle progression in oral squamous cell carcinoma cell lines[J]. ORAL DISEASES,2015,21(2):156-162.
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