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学科主题临床医学
The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells
Wu, Pengjie1; Zhang, Ning2; Wang, Xi1; Zhang, Chi3; Li, Teng1; Ning, Xianghui1; Gong, Kan1
刊名PLOS ONE
2012-09-18
DOI10.1371/journal.pone.0045122
7期:9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]TUMOR-SUPPRESSOR GENE ; HYPOXIA-INDUCIBLE FACTORS ; HIPPEL-LINDAU-DISEASE ; ERYTHROPOIETIN RECEPTOR ; EXPRESSION ; COEXPRESSION ; HEMANGIOBLASTOMA ; PROLIFERATION ; ACTIVATION ; MUTATIONS
英文摘要

Co-expression of erythropoietin (Epo) and erythropoietin receptor (EpoR) has been found in various non-hematopoietic cancers including hereditary and sporadic renal cell carcinomas (RCC), but the Epo/EpoR autocrine and paracrine mechanisms in tumor progression have not yet been identified. In this study, we used RNA interference method to down-regulate EpoR to investigate the function of Epo/EpoR pathway in human RCC cells. Epo and EpoR co-expressed in primary renal cancer cells and 6 human RCC cell lines. EpoR signaling was constitutionally phosphorylated in primary renal cancer cells, 786-0 and Caki-1 cells, and recombinant human Epo (rhEpo) stimulation had no significant effects on further phosphorylation of EpoR pathway, proliferation, and invasiveness of the cells. Down-regulation of EpoR expression in 7860 cells by lentivirus-introduced siRNA resulted in inhibition of growth and invasiveness in vitro and in vivo, and promotion of cell apoptosis. In addition, rhEpo stimulation slightly antagonized the anti-tumor effect of Sunitinib on 786-0 cells. Sunitinib could induce more apoptotic cells in 786-0 cells with knockdown EpoR expression. Our results suggested that Epo/EpoR pathway was involved in cell growth, invasion, survival, and sensitivity to the multi-kinases inhibitor Sunitinib in RCC cells.

语种英语
WOS记录号WOS:000311313900082
项目编号30872560 ; 81172418
资助机构National Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54131
专题北京大学第一临床医学院_泌尿外科
北京大学第三临床医学院_骨科
北京大学第三临床医学院_信息中心
作者单位1.Peking Univ, Natl Urol Canc Ctr, Dept Urol, Inst Urol,Hosp 1, Beijing 100871, Peoples R China
2.Capital Univ Med Sci, Dept Urol, Beijing Chaoyang Hosp, Beijing, Peoples R China
3.Peking Univ, Dept Gen Surg, Hosp 1, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wu, Pengjie,Zhang, Ning,Wang, Xi,et al. The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells[J]. PLOS ONE,2012,7(9).
APA Wu, Pengjie.,Zhang, Ning.,Wang, Xi.,Zhang, Chi.,Li, Teng.,...&Gong, Kan.(2012).The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells.PLOS ONE,7(9).
MLA Wu, Pengjie,et al."The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells".PLOS ONE 7.9(2012).
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