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学科主题: 临床医学
题名:
The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells
作者: Wu, Pengjie1; Zhang, Ning2; Wang, Xi1; Zhang, Chi3; Li, Teng1; Ning, Xianghui1; Gong, Kan1
刊名: PLOS ONE
发表日期: 2012-09-18
DOI: 10.1371/journal.pone.0045122
卷: 7, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: TUMOR-SUPPRESSOR GENE ; HYPOXIA-INDUCIBLE FACTORS ; HIPPEL-LINDAU-DISEASE ; ERYTHROPOIETIN RECEPTOR ; EXPRESSION ; COEXPRESSION ; HEMANGIOBLASTOMA ; PROLIFERATION ; ACTIVATION ; MUTATIONS
英文摘要:

Co-expression of erythropoietin (Epo) and erythropoietin receptor (EpoR) has been found in various non-hematopoietic cancers including hereditary and sporadic renal cell carcinomas (RCC), but the Epo/EpoR autocrine and paracrine mechanisms in tumor progression have not yet been identified. In this study, we used RNA interference method to down-regulate EpoR to investigate the function of Epo/EpoR pathway in human RCC cells. Epo and EpoR co-expressed in primary renal cancer cells and 6 human RCC cell lines. EpoR signaling was constitutionally phosphorylated in primary renal cancer cells, 786-0 and Caki-1 cells, and recombinant human Epo (rhEpo) stimulation had no significant effects on further phosphorylation of EpoR pathway, proliferation, and invasiveness of the cells. Down-regulation of EpoR expression in 7860 cells by lentivirus-introduced siRNA resulted in inhibition of growth and invasiveness in vitro and in vivo, and promotion of cell apoptosis. In addition, rhEpo stimulation slightly antagonized the anti-tumor effect of Sunitinib on 786-0 cells. Sunitinib could induce more apoptotic cells in 786-0 cells with knockdown EpoR expression. Our results suggested that Epo/EpoR pathway was involved in cell growth, invasion, survival, and sensitivity to the multi-kinases inhibitor Sunitinib in RCC cells.

语种: 英语
所属项目编号: 30872560 ; 81172418
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000311313900082
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54131
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Natl Urol Canc Ctr, Dept Urol, Inst Urol,Hosp 1, Beijing 100871, Peoples R China
2.Capital Univ Med Sci, Dept Urol, Beijing Chaoyang Hosp, Beijing, Peoples R China
3.Peking Univ, Dept Gen Surg, Hosp 1, Beijing 100871, Peoples R China

Recommended Citation:
Wu, Pengjie,Zhang, Ning,Wang, Xi,et al. The Erythropoietin/Erythropoietin Receptor Signaling Pathway Promotes Growth and Invasion Abilities in Human Renal Carcinoma Cells[J]. PLOS ONE,2012,7(9).
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