IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
Immunolocalization of UDP-glucose : glycoprotein glucosyltransferase indicates involvement of pre-Golgi intermediates in protein quality control
Zuber, C; Fan, JY; Guhl, B; Parodi, A; Fessler, JH; Parker, C; Roth, J
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2001-09-11
98期:19页:10710-10715
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]ULTRATHIN FROZEN-SECTIONS ; CLASS-I MOLECULES ; ENDOPLASMIC-RETICULUM ; SECRETORY PATHWAY ; LINKED OLIGOSACCHARIDES ; ER ; CALNEXIN ; RECOGNITION ; CHAPERONE ; RETENTION
英文摘要

The UDP-glucose:glycoprotein glucosyltransferase (GT) is a protein folding sensor and glycosyltransferase that constitutes an important component of the protein quality control machinery. With the use of quantitative immunogold electron microscopy, we established the subcellular distribution of GT in rat liver and pancreas and Drosophila melanogaster salivary gland as well as cell lines and correlated it with that of glucosidase II, calreticulin, a nd pre-Golgi intermediate markers. Labeling for GT, as well as for glucosidase II and calreticulin, was found in the endoplasmic reticulum (ER), including nuclear envelope and pre-Golgi intermediates located between ER and Golgi apparatus, and in the cell periphery. In the rough ER, labeling for GT was inhomogeneous, with variously sized labeled and unlabeled cisternal regions alternating, indicative of a meshwork of quality control checkpoints. Notably, labeling intensity for GT was highest in pre-Golgi intermediates, corresponding to twice that of rough ER, whereas the Golgi apparatus exhibited no specific labeling. These results suggest that protein quality control is not restricted to the ER and that the pre-Golgi intermediates, by virtue of the presence of GT, glucosidase II, and calreticulin, are involved in this fundamental cellular process.

语种英语
WOS记录号WOS:000170966800040
Citation statistics
Cited Times:69[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54133
Collection北京大学基础医学院_生物物理学系
作者单位1.Beijing Univ, Dept Biophys, Beijing 100083, Peoples R China
2.Univ Zurich, Dept Pathol, Div Cell & Mol Pathol, CH-8091 Zurich, Switzerland
3.Univ San Martin, Inst Invest Biotecnol, RA-1650 Buenos Aires, DF, Argentina
4.Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA
5.Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA
6.Univ Calif Los Angeles, Mol Cellular & Dev Biol Dept, Los Angeles, CA 90095 USA
Recommended Citation
GB/T 7714
Zuber, C,Fan, JY,Guhl, B,et al. Immunolocalization of UDP-glucose : glycoprotein glucosyltransferase indicates involvement of pre-Golgi intermediates in protein quality control[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2001,98(19):10710-10715.
APA Zuber, C.,Fan, JY.,Guhl, B.,Parodi, A.,Fessler, JH.,...&Roth, J.(2001).Immunolocalization of UDP-glucose : glycoprotein glucosyltransferase indicates involvement of pre-Golgi intermediates in protein quality control.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,98(19),10710-10715.
MLA Zuber, C,et al."Immunolocalization of UDP-glucose : glycoprotein glucosyltransferase indicates involvement of pre-Golgi intermediates in protein quality control".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 98.19(2001):10710-10715.
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