IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
SNIPER: A Novel Assay for Human Papillomavirus Testing Among Women in Guizhou, China
Belinson, Suzanne E.1; Wulan, Na2; Li, Ruizhen2; Zhang, Wei2; Rong, Xuan2; Zhu, Yasha2; Wu, Ruifang2; Belinson, Jerome L.1
关键词Human Papillomavirus Assay Cervical Cancer Screening
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2010-08-01
DOI10.1111/IGC.0b013e3181e73092
20期:6页:1006-1010
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]CERVICAL-CANCER ; HPV ; CYTOLOGY ; RISK ; EXPERIENCE ; TRIAGE ; DNA
英文摘要

Objective: Clinically validate the SNIPER human papillomavirus (HPV) DNA assay for the detection of cervical intraepithelial neoplasia (CIN) 2 or higher and CIN2 or higher in a prospective cross-sectional screening study in Guizhou Province, China.

Methods: Between March and April, 2008, 1000 nonpregnant women aged 30 or older were recruited in Guizhou Province, China. Women positive by SNIPER or cytological examination were requested to return for follow-up. A biopsy of all colposcopically detected abnormalities was performed by quadrant. In normal quadrants, biopsies were obtained at the squamocolumnar junction (2-, 4-, 8-, and 10-o′clock positions depending on the quadrant). Samples were placed in 2 mL of saline solution and maintained between 2 degrees C and 30 degrees C for up to 1 week. One milliliter of this suspension was then prepared and tested. For polymerase chain reaction amplification, a pool of HPV primers was designed to amplify HPV DNA from 13 high-risk-HPV genotypes (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68). Test characteristics were calculated according to standard definitions.

Results: One thousand women were screened; 175 tested HPV positive, 36 women tested negative but had positive Papanicolaou test results. All but 21 (90%) returned for follow-up. Median age and proportions having CIN2 or higher and CIN3 or higher differed by HPV status. Twenty-five women had CIN2 or higher and 16 had CIN3 or higher. The SNIPER assay was 93.3% and 94% sensitive and 86% and 85% specific for the detection of CIN2 or higher and CIN3 or higher, respectively. The positive predictive value was 17.4% and 9.9% for CIN2 or higher and CIN3 or higher, respectively. Negative predictive value approached 100% for CIN2 or higher and CIN3 or higher.

Conclusion: The SNIPER assay is functionally competitive and in terms of cost holds an advantage over Hybrid Capture 2 in a Chinese healthcare market, and potentially others, around the world.

语种英语
WOS记录号WOS:000280572000015
项目编号R25 CA100600
资助机构National Cancer Institute
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54139
专题北京大学深圳医院
作者单位1.Prevent Oncol Int Inc, Cleveland, OH 44118 USA
2.Beijing Univ, Dept Obstet & Gynecol, Shenzhen Hosp, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Belinson, Suzanne E.,Wulan, Na,Li, Ruizhen,et al. SNIPER: A Novel Assay for Human Papillomavirus Testing Among Women in Guizhou, China[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2010,20(6):1006-1010.
APA Belinson, Suzanne E..,Wulan, Na.,Li, Ruizhen.,Zhang, Wei.,Rong, Xuan.,...&Belinson, Jerome L..(2010).SNIPER: A Novel Assay for Human Papillomavirus Testing Among Women in Guizhou, China.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,20(6),1006-1010.
MLA Belinson, Suzanne E.,et al."SNIPER: A Novel Assay for Human Papillomavirus Testing Among Women in Guizhou, China".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 20.6(2010):1006-1010.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Belinson, Suzanne E.]的文章
[Wulan, Na]的文章
[Li, Ruizhen]的文章
百度学术
百度学术中相似的文章
[Belinson, Suzanne E.]的文章
[Wulan, Na]的文章
[Li, Ruizhen]的文章
必应学术
必应学术中相似的文章
[Belinson, Suzanne E.]的文章
[Wulan, Na]的文章
[Li, Ruizhen]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。