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学科主题: 临床医学
题名:
Functional Expression of Ropporin in Human Testis and Ejaculated Spermatozoa
作者: Chen, Jing1; Wang, Yong1; Wei, Benlin2; Lai, Yongqing2; Yan, Qiuxia1; Gui, Yaoting1; Cai, Zhiming1
关键词: microarray analysis ; asthenozoospermia ; spermatozoa motility ; Rho
刊名: JOURNAL OF ANDROLOGY
发表日期: 2011
DOI: 10.2164/jandrol.109.009662
卷: 32, 期:1, 页:26-32
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Andrology
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: SPERM MOTILITY ; ASTHENOZOOSPERMIC MEN ; FIBROUS SHEATH ; PROTEINS ; RNA ; IDENTIFICATION
英文摘要:

Asthenozoospermia is a common cause of human male infertility, but the molecular mechanism is not fully understood. With Affymetrix Genechips, ropporin, a component of sperm flagella, was identified by comparing the expression profiles in ejaculated spermatozoa from normozoospermic men and patients with asthenozoospermia. Immunohistochemistry was used to analyze the expression characteristic of ropporin in human testis. Reverse transcription-polymerase chain reaction, Western blotting, and indirect immunofluorescence assay were used to determine the expression of ropporin in ejaculated spermatozoa from normozoospermic and asthenozoospermic men. The results showed that ropporin was predominantly expressed in round spermatids in human testis, and located in the principal piece and the end piece of spermatozoa flagella. The expression level of ropporin was significantly lower in asthenozoospermic men than in normozoospermic controls. These data suggested that ropporin may be involved in sperm motility and its decreased expression may contribute to the low sperm motility in asthenozoospernnic patients.

语种: 英语
所属项目编号: 30770810 ; 30700824 ; 30972992 ; 200903078
项目资助者: National Natural Science Foundation of China ; Science Foundation of Shenzhen, China
WOS记录号: WOS:000286286700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54147
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen 518036, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen 518036, Peoples R China

Recommended Citation:
Chen, Jing,Wang, Yong,Wei, Benlin,et al. Functional Expression of Ropporin in Human Testis and Ejaculated Spermatozoa[J]. JOURNAL OF ANDROLOGY,2011,32(1):26-32.
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