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学科主题: 公共卫生
题名:
The role of MAPK in the biphasic dose-response phenomenon induced by cadmium and mercury in HEK293 cells
作者: Hao, Changfu; Hao, Weidong; Wei, Xuetao; Xing, Lina; Jiang, Jianjun; Shang, Lanqin
关键词: Biphasic dose-response ; Cadmium ; Mercury ; Mitogen-activated protein kinase ; Proliferation ; Apoptosis
刊名: TOXICOLOGY IN VITRO
发表日期: 2009-06-01
DOI: 10.1016/j.tiv.2009.03.005
卷: 23, 期:4, 页:660-666
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: GROWTH-INHIBITION ; STRESS-RESPONSE ; KINASE CASCADE ; HORMESIS ; APOPTOSIS ; METALS ; PATHWAY ; ACTIVATION ; TOLERANCE ; EXPOSURE
英文摘要:

Some studies show that Cd(2+) and Hg(2+) may induce cell proliferation and apoptosis via biphasic dose-response relationship in human cells. However, mechanisms underlying this phenomenon are still in puzzle. In this study, we aim at detecting the biphasic effects of Cd(2+) and Hg(2+) on proliferation and apoptosis of human embryonic kidney 293 (HEK293) cells, analyzing the change of the mitogen-activated protein kinase (MAPK) pathways, and discussing the relationship between them. The results demonstrate that Cd(2+) and Hg(2+) can stimulate cell proliferation at lower concentrations (0.05 and 0.5 mu M) but inhibit it at higher concentrations (50 and 500 mu M). Apoptosis increases at higher concentrations (50 and 500 mu M) of Cd(2+) and Hg(2+). While 0.5 mu M Cd(2+) and Hg(2+) decrease the JNK phosphorylation, 50 mu M Cd(2+) and Hg(2+) increase the JNK and P38 phosphorylation. When HEK293 cells are treated with 20 mu M JNK inhibitor or 100 mu M ERK1/2 inhibitor, the cell proliferation do not increase significantly at low concentrations (0.05 and 0.5 mu M), but still decrease at high concentrations (50 and 500 mu M). When HEK293 cells are treated with 20 mu M P38 inhibitor, the tendency of cell proliferation is not affected. Data in our study suggests that activation of MAPK pathway may be involved in the biphasic effect induced by Cd(2+) and Hg(2+). (C) 2009 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 5062025
项目资助者: Natural Science Foundation of Beijing city
WOS记录号: WOS:000267011400017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54151
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Hao, Changfu,Hao, Weidong,Wei, Xuetao,et al. The role of MAPK in the biphasic dose-response phenomenon induced by cadmium and mercury in HEK293 cells[J]. TOXICOLOGY IN VITRO,2009,23(4):660-666.
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