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Clinical Evaluation of a New Quantitative Enzyme-Linked Immunosorbent Assay for Anti-dsDNA Antibodies Measured in Patients With Systemic Lupus Erythematosus
Cui, Liyan; Zhang, Jie; Hu, Xiaozhou; Zhang, Wenjing; Ding, Jie
关键词Immunology Ap Technique
刊名LABMEDICINE
2010-12-01
DOI10.1309/LMLJNSSXX4PC37PL
41期:12页:724-727
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]AUTOANTIBODIES ; SPECIFICITY ; PREDICTORS
英文摘要

Background The measurement of autoantibodies specific for double stranded DNA (anti dsDNA) is a useful tool for the diagnosis and prognosis of systemic lupus erythematosus (SLE) A quantitative enzyme linked immunosorbent assay (ELISA) for anti dsDNA antibodies has recently become available for the determination of anti dsDNA antibodies The aim of this study was to evaluate the clinical performance characteristics of this assay in patients with inactive or active SLE

Methods Serum samples from patients with inactive (n=65) or active (n=112) SLE were tested for anti dsDNA autoantibodies using 2 methods from Euroimmun AG (Lubeck, Germany) a new ELISA anti dsDNA assay and the currently available Crithidia luciliae indirect immunofluorescent test (CLIFT) In addition, the levels of the complement components C3 and C4 biological markers associated with SLE disease activity were measured by nephelometry in the sera from all patients

Results The sensitivity and specificity of the anti dsDNA ELISA assay for discriminating between patients with inactive or active SLE were 93 54% and 67 69% respectively Among all patients (n=177) there was a weak concordance (kappa=0 264) between anti dsDNA results by the ELISA and CLIFT assay However a significant inverse correlation was found with complement components levels In addition, area under the curve (AUC) values for anti dsDNA as well as C3 and C4 assays decreased in the following order 0 901 (ELISA for anti dsDNA autoantibodies)>0 734 (C3)>0 723 (C4)>0 650 (CLIFT for anti dsDNA autoantibodies)

Conclusions Our results show the ELISA for anti dsDNA autoantibodies is significantly more sensitive and specific than any of the other assays we evaluated for discriminating between patients with inactive or active SLE Moreover, the inverse correlation between ELISA anti dsDNA autoantibody and complement levels suggests that anti dsDNA results by the Euroimmun ELISA may be promising for monitoring disease activity in patients with SLE

语种英语
WOS记录号WOS:000284681700004
资助机构EU-ROIMMUN Medicine Diagnostics AG (Lubeck, Germany), China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54158
专题北京大学第三临床医学院
北京大学第三临床医学院_检验科
作者单位Peking Univ, Dept Lab Med, Hosp 3, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Cui, Liyan,Zhang, Jie,Hu, Xiaozhou,et al. Clinical Evaluation of a New Quantitative Enzyme-Linked Immunosorbent Assay for Anti-dsDNA Antibodies Measured in Patients With Systemic Lupus Erythematosus[J]. LABMEDICINE,2010,41(12):724-727.
APA Cui, Liyan,Zhang, Jie,Hu, Xiaozhou,Zhang, Wenjing,&Ding, Jie.(2010).Clinical Evaluation of a New Quantitative Enzyme-Linked Immunosorbent Assay for Anti-dsDNA Antibodies Measured in Patients With Systemic Lupus Erythematosus.LABMEDICINE,41(12),724-727.
MLA Cui, Liyan,et al."Clinical Evaluation of a New Quantitative Enzyme-Linked Immunosorbent Assay for Anti-dsDNA Antibodies Measured in Patients With Systemic Lupus Erythematosus".LABMEDICINE 41.12(2010):724-727.
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