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学科主题: 临床医学
题名:
ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats
作者: Ni, Hong1; Jiang, Yu-wu2; Tao, Lu-yang1; Jin, Mei-fang1; Wu, Xi-ru2
关键词: Zinc transporter ; CaMK II ; PRG-1 ; Seizure ; Neonatal ; Cognition
刊名: TOXICOLOGY LETTERS
发表日期: 2009-02-10
DOI: 10.1016/j.toxlet.2008.11.003
卷: 184, 期:3, 页:145-150
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: RECEPTOR EXPRESSION ; WISTAR RAT ; MEMORY ; BRAIN ; ZINC ; TRAFFICKING
英文摘要:

Epilepsy in children is associated with abroad spectrum of cognitive deficits, which is associated with hippocampal mossy fiber sprouting. The underlying molecular mechanisms involved in mossy fiber sprouting in hippocampus following developmental seizures are not completely known. We studied the timing of cognitive dysfunction following neonatal seizures and the relation of this cognitive impairment to zinc transporter I (ZnT-1), 3 (ZnT-3), calcium/calmodulin-dependent protein kinase II (CaMK II), plasticity-related gene 1 (PRG-1) expression in hippocampus. A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6). Rats were assigned into the single-seizure group (SS), the recurrent-seizure group (RS, seizures induced in six consecutive days), and the control group. During P41-P46 and P85-P90, the rats were tested for spatial learning and memory abilities with automatic Morris water maze task. At P90, mossy fiber sprouting and gene expression in hippocampus were determined subsequently by Timm staining and RT-PCR methods. The escape latencies from the water maze were significantly longer in rats of RS group than those of the control and SS groups at d4 of the first maze test and at d3, d4 of the second maze test. As far as Spatial Probe Test was concerned, the frequency of passing through the platform quadrant was significantly decreased in RS group than that in control and SS groups in the entire two probe tests. in rats with recurrent seizures (RS group), there was an increased distribution of Timm granules in both the supragranular region of the dentate gyrus and the stratum pyramidale of CA3 subfield in RS group, while remaining barely visible in control and SS groups: the Timm scores in CA3 and dentate gyrus in the RS animals were significantly higher than that in the control and SS groups. RT-PCR densitometry analysis showed that the ratios of hippocampal ZnT-1 to beta-actin of SS and IRS group were decreased significantly compared with that of control group. Meanwhile, CaMK II to beta-actin of RS group was markedly lower compared with those of SS and control groups. Our results suggest that the long-term adverse effects of recurrent neonatal seizures on cognition and mossy fiber sprouting may be associated with the down-regulated expression of ZnT-1 and CaMK II in hippocampus. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 30470555 ; 30571909 ; 30870808 ; 30872666 ; 2003034094 ; BK2007509 ; 07KJB320103509
项目资助者: National Natural Science Foundation of China ; 34th Postdoctoral Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China
WOS记录号: WOS:000263382500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54164
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Soochow Univ, Childrens Hosp, Neurol Lab, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Ni, Hong,Jiang, Yu-wu,Tao, Lu-yang,et al. ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats[J]. TOXICOLOGY LETTERS,2009,184(3):145-150.
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