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学科主题临床医学
ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats
Ni, Hong1; Jiang, Yu-wu2; Tao, Lu-yang1; Jin, Mei-fang1; Wu, Xi-ru2
关键词Zinc Transporter Camk Ii Prg-1 Seizure Neonatal Cognition
刊名TOXICOLOGY LETTERS
2009-02-10
DOI10.1016/j.toxlet.2008.11.003
184期:3页:145-150
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]RECEPTOR EXPRESSION ; WISTAR RAT ; MEMORY ; BRAIN ; ZINC ; TRAFFICKING
英文摘要

Epilepsy in children is associated with abroad spectrum of cognitive deficits, which is associated with hippocampal mossy fiber sprouting. The underlying molecular mechanisms involved in mossy fiber sprouting in hippocampus following developmental seizures are not completely known. We studied the timing of cognitive dysfunction following neonatal seizures and the relation of this cognitive impairment to zinc transporter I (ZnT-1), 3 (ZnT-3), calcium/calmodulin-dependent protein kinase II (CaMK II), plasticity-related gene 1 (PRG-1) expression in hippocampus. A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6). Rats were assigned into the single-seizure group (SS), the recurrent-seizure group (RS, seizures induced in six consecutive days), and the control group. During P41-P46 and P85-P90, the rats were tested for spatial learning and memory abilities with automatic Morris water maze task. At P90, mossy fiber sprouting and gene expression in hippocampus were determined subsequently by Timm staining and RT-PCR methods. The escape latencies from the water maze were significantly longer in rats of RS group than those of the control and SS groups at d4 of the first maze test and at d3, d4 of the second maze test. As far as Spatial Probe Test was concerned, the frequency of passing through the platform quadrant was significantly decreased in RS group than that in control and SS groups in the entire two probe tests. in rats with recurrent seizures (RS group), there was an increased distribution of Timm granules in both the supragranular region of the dentate gyrus and the stratum pyramidale of CA3 subfield in RS group, while remaining barely visible in control and SS groups: the Timm scores in CA3 and dentate gyrus in the RS animals were significantly higher than that in the control and SS groups. RT-PCR densitometry analysis showed that the ratios of hippocampal ZnT-1 to beta-actin of SS and IRS group were decreased significantly compared with that of control group. Meanwhile, CaMK II to beta-actin of RS group was markedly lower compared with those of SS and control groups. Our results suggest that the long-term adverse effects of recurrent neonatal seizures on cognition and mossy fiber sprouting may be associated with the down-regulated expression of ZnT-1 and CaMK II in hippocampus. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000263382500001
项目编号30470555 ; 30571909 ; 30870808 ; 30872666 ; 2003034094 ; BK2007509 ; 07KJB320103509
资助机构National Natural Science Foundation of China ; 34th Postdoctoral Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54164
专题北京大学第一临床医学院_儿科
作者单位1.Soochow Univ, Childrens Hosp, Neurol Lab, Lab Aging & Nervous Dis, Suzhou 215003, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Ni, Hong,Jiang, Yu-wu,Tao, Lu-yang,et al. ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats[J]. TOXICOLOGY LETTERS,2009,184(3):145-150.
APA Ni, Hong,Jiang, Yu-wu,Tao, Lu-yang,Jin, Mei-fang,&Wu, Xi-ru.(2009).ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats.TOXICOLOGY LETTERS,184(3),145-150.
MLA Ni, Hong,et al."ZnT-1, ZnT-3, CaMK II, PRG-1 expressions in hippocampus following neonatal seizure-induced cognitive deficit in rats".TOXICOLOGY LETTERS 184.3(2009):145-150.
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