IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1)
Gao, Wen-long1; Liu, Ming1; Yang, Yanyan1,4; Yang, Huixia2; Liao, Qinping2; Bai, Yang1,4; Li, Yu-xia1; Li, Dong1,4; Peng, Chun3; Wang, Yan-Ling1
关键词H19 Mir-675 Nomo1 Placental Trophoblast Cell Proliferation Preeclampsia
刊名RNA BIOLOGY
2012-07-01
DOI10.4161/rna.20807
9期:7页:1002-1010
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者Chinese National Special Fund for Basic Research ; National Natural Sciences Foundation ; Knowledge Innovation Program in Chinese Academy of Sciences ; Chinese National Special Fund for Basic Research ; National Natural Sciences Foundation ; Knowledge Innovation Program in Chinese Academy of Sciences
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]NONCODING RNA ; SOLUBLE ENDOGLIN ; DNA METHYLATION ; PREECLAMPSIA ; MICRORNAS ; BINDING ; CYTOTROPHOBLASTS ; INTERFERENCE ; PATHOGENESIS ; EXPRESSION
英文摘要

Preeclampsia is a pregnancy-specific syndrome mainly characterized by hypertensive disorder and proteinuria after gestational weeks 20. So far the etiology of preeclampsia remains unclear. We previously reported that preeclamptic placentas exhibited decreased mRNA expression and hypermethylation in promoter region of the paternally imprinted H19 gene compared with normal placentas. H19 has recently been identified to encode the precursor of miR-675, indicating a possible novel functional pathway of the imprinting gene. The aim of the present study was to identify the roles of H19 gene via miR-675 pathway in human trophoblast cells, and to figure out the involvement of this pathway in pathogenesis of preeclampsia. Knockdown of H19 gene or inhibition of miR-675 exhibited similar proliferation-promoting effect in human trophoblastic JEG-3 cells. Target gene prediction in combination with luciferase assay revealed that miR-675 could directly down-regulate Nodal Modulator 1 (NOMO1) protein expression by binding to 3′-UTR sequence of NOMO1. Overexpression of NOMO1 in JEG-3 cells could rescue miR-675-surppressed cell proliferation and phosphorylation of Smad2, while Nodal had additive effect with miR-675 in suppression cell proliferation and activation of Smad2. In early-onset preeclamptic placentas, expression levels of H19 gene and miR-675 were appreciably lower, while NOMO1 protein level was higher than those in normal placentas. Taken together, our data suggested that H19 gene could inhibit human trophoblast cell proliferation via encoding miR-675 that targeted NOMO1, and aberrantly lowered expression of H19 in placenta may participate in the excessive proliferation of trophoblast cells observed in early-onset severe preeclampsia by downregulating miR-675 which targets NOMO1 and interferes with Nodal signaling.

语种英语
所属项目编号2011CB944404 ; 81025004 ; 30811120430 ; KSCX2-EW-R-06
资助者Chinese National Special Fund for Basic Research ; National Natural Sciences Foundation ; Knowledge Innovation Program in Chinese Academy of Sciences ; Chinese National Special Fund for Basic Research ; National Natural Sciences Foundation ; Knowledge Innovation Program in Chinese Academy of Sciences
WOS记录号WOS:000307078700011
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54166
专题北京大学第一临床医学院
作者单位1.York Univ, Dept Biol, Toronto, ON M3J 2R7, Canada
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Gao, Wen-long,Liu, Ming,Yang, Yanyan,et al. The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1)[J]. RNA BIOLOGY,2012,9(7):1002-1010.
APA Gao, Wen-long.,Liu, Ming.,Yang, Yanyan.,Yang, Huixia.,Liao, Qinping.,...&Wang, Yan-Ling.(2012).The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1).RNA BIOLOGY,9(7),1002-1010.
MLA Gao, Wen-long,et al."The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1)".RNA BIOLOGY 9.7(2012):1002-1010.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Gao, Wen-long]的文章
[Liu, Ming]的文章
[Yang, Yanyan]的文章
百度学术
百度学术中相似的文章
[Gao, Wen-long]的文章
[Liu, Ming]的文章
[Yang, Yanyan]的文章
必应学术
必应学术中相似的文章
[Gao, Wen-long]的文章
[Liu, Ming]的文章
[Yang, Yanyan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。