北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 期刊论文
学科主题: 临床医学
题名:
The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1)
作者: Gao, Wen-long1; Liu, Ming1; Yang, Yanyan1,4; Yang, Huixia2; Liao, Qinping2; Bai, Yang1,4; Li, Yu-xia1; Li, Dong1,4; Peng, Chun3; Wang, Yan-Ling1
关键词: H19 ; miR-675 ; NOMO1 ; placental trophoblast ; cell proliferation ; preeclampsia
刊名: RNA BIOLOGY
发表日期: 2012-07-01
DOI: 10.4161/rna.20807
卷: 9, 期:7, 页:1002-1010
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NONCODING RNA ; SOLUBLE ENDOGLIN ; DNA METHYLATION ; PREECLAMPSIA ; MICRORNAS ; BINDING ; CYTOTROPHOBLASTS ; INTERFERENCE ; PATHOGENESIS ; EXPRESSION
英文摘要:

Preeclampsia is a pregnancy-specific syndrome mainly characterized by hypertensive disorder and proteinuria after gestational weeks 20. So far the etiology of preeclampsia remains unclear. We previously reported that preeclamptic placentas exhibited decreased mRNA expression and hypermethylation in promoter region of the paternally imprinted H19 gene compared with normal placentas. H19 has recently been identified to encode the precursor of miR-675, indicating a possible novel functional pathway of the imprinting gene. The aim of the present study was to identify the roles of H19 gene via miR-675 pathway in human trophoblast cells, and to figure out the involvement of this pathway in pathogenesis of preeclampsia. Knockdown of H19 gene or inhibition of miR-675 exhibited similar proliferation-promoting effect in human trophoblastic JEG-3 cells. Target gene prediction in combination with luciferase assay revealed that miR-675 could directly down-regulate Nodal Modulator 1 (NOMO1) protein expression by binding to 3′-UTR sequence of NOMO1. Overexpression of NOMO1 in JEG-3 cells could rescue miR-675-surppressed cell proliferation and phosphorylation of Smad2, while Nodal had additive effect with miR-675 in suppression cell proliferation and activation of Smad2. In early-onset preeclamptic placentas, expression levels of H19 gene and miR-675 were appreciably lower, while NOMO1 protein level was higher than those in normal placentas. Taken together, our data suggested that H19 gene could inhibit human trophoblast cell proliferation via encoding miR-675 that targeted NOMO1, and aberrantly lowered expression of H19 in placenta may participate in the excessive proliferation of trophoblast cells observed in early-onset severe preeclampsia by downregulating miR-675 which targets NOMO1 and interferes with Nodal signaling.

语种: 英语
所属项目编号: 2011CB944404 ; 81025004 ; 30811120430 ; KSCX2-EW-R-06
项目资助者: Chinese National Special Fund for Basic Research ; National Natural Sciences Foundation ; Knowledge Innovation Program in Chinese Academy of Sciences
WOS记录号: WOS:000307078700011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54166
Appears in Collections:北京大学第一临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.York Univ, Dept Biol, Toronto, ON M3J 2R7, Canada
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
4.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R China

Recommended Citation:
Gao, Wen-long,Liu, Ming,Yang, Yanyan,et al. The imprinted H19 gene regulates human placental trophoblast cell proliferation via encoding miR-675 that targets Nodal Modulator 1 (NOMO1)[J]. RNA BIOLOGY,2012,9(7):1002-1010.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, Wen-long]'s Articles
[Liu, Ming]'s Articles
[Yang, Yanyan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, Wen-long]‘s Articles
[Liu, Ming]‘s Articles
[Yang, Yanyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace