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学科主题: 临床医学
题名:
Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts
作者: Yu Ming-ming1; Wang Rong-fu2; Chen Yue-hua1; Zhou Hai-zhong1; Deng Xiao-hu1
关键词: LyP-1 peptide ; MDA-MB-435 xenografts ; biodistribution ; imaging
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-02-05
DOI: 10.3760/cma.j.issn.0366-6999.20112546
卷: 126, 期:3, 页:471-475
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HOMING PEPTIDE ; P-32 PROTEIN ; TUMOR-CELLS ; METASTASIS ; HYALURONAN ; EXPRESSION
英文摘要:

Background Recent studies have shown the LyP-1 peptide can home to either tumor lymphatics or the tumor cells and be internalized by targeted cells. This study aimed to investigate the possibility of using (NaI)-I-131 labeled LyP-1 peptide as an imaging agent or a therapeutic radiopharmaceutical in breast carcinoma and its metastasis.

Methods The 10-mer cyclic peptide contained the LyP-1 sequence (YCGNKRTRGC) was synthesized by the solid phase method. Disulfide bonds between the cysteines maintain the cyclic structure. The LyP-1 peptide was labeled with (NaI)-I-131 using the chloramine-T method. The [I-131] LyP-1 peptide and a [I-131] control peptide were injected via tail vein into nude mice bearing MDA-MB-435 tumor xenografts. Biodistribution and imaging results in vivo were obtained.

Results The labeling efficiencies of LyP-1 peptide reached 80% +/- 5% (n=5). The radiochemical purity was about 96%. The radiochemical purity of the labeled compound remains 92% at 24 hours in human serum at 37 degrees C. In the biodistribution studies, the [I-131] LyP-1 peptide accumulated in the tumor to a higher level than in other organs. The [I-131] LyP-1 peptide can successfully image the tumor in nude mice bearing MDA-MB-435 tumor xenografts.

Conclusions The LyP-1 peptide could be effectively labeled with (NaI)-I-131 and the labeled compound is stable in human serum at 37 degrees C for 24 hours. The high specificity of [I-131] LyP-1 peptide suggests it may be a promising new radiotracer for identifying tumors.

语种: 英语
WOS记录号: WOS:000319632900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54182
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

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作者单位: 1.Yangzhou Univ, Clin Med Coll, Dept Nucl Med, Yangzhou 225001, Jiangsu, Peoples R China
2.Peking Univ, Hosp 1, Dept Nucl Med, Beijing 100034, Peoples R China

Recommended Citation:
Yu Ming-ming,Wang Rong-fu,Chen Yue-hua,et al. Radiolabeling LyP-1 peptide and preliminary biodistribution evaluation in mice bearing MDA-MB-435 xenografts[J]. CHINESE MEDICAL JOURNAL,2013,126(3):471-475.
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