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学科主题临床医学
Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection
Gao, Fei; Dong, Juan; Li, Wei; Wang, Tao; Liao, Jie; Liao, Yiping; Liu, Huwei
关键词Capillary Zone Electrophoresis Phospholipids Separation Indirect Uv Detection
刊名JOURNAL OF CHROMATOGRAPHY A
2006-10-20
DOI10.1016/j.chroma.2006.03.070
1130期:2页:259-264
收录类别ISTP ; SCI
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; INDIRECT PHOTOMETRIC DETECTION ; LIGHT-SCATTERING DETECTION ; MASS-SPECTROMETRY ; LIPIDS
英文摘要

A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000241218300016
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54191
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Hostp 1, Inst Nephrol, Beijing 100034, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Dept Chem, Minist Educ,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Med Expt & Anal Ctr, Beijing 100853, Peoples R China
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GB/T 7714
Gao, Fei,Dong, Juan,Li, Wei,et al. Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1130(2):259-264.
APA Gao, Fei.,Dong, Juan.,Li, Wei.,Wang, Tao.,Liao, Jie.,...&Liu, Huwei.(2006).Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.JOURNAL OF CHROMATOGRAPHY A,1130(2),259-264.
MLA Gao, Fei,et al."Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection".JOURNAL OF CHROMATOGRAPHY A 1130.2(2006):259-264.
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