|A Host Defense Mechanism Involving CFTR-Mediated Bicarbonate Secretion in Bacterial Prostatitis|
|Xie, Chen1; Tang, Xiaoxiao1; Xu, Wenming2; Diao, Ruiying3; Cai, Zhiming3; Chan, Hsiao Chang1,2|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||TRANSMEMBRANE CONDUCTANCE REGULATOR ; SOLUBLE ADENYLYL-CYCLASE ; CONGENITAL CHLORIDE DIARRHEA ; CYSTIC-FIBROSIS ; FERTILIZING-CAPACITY ; CL-/HCO3-EXCHANGE ; ESCHERICHIA-COLI ; MESSENGER-RNA ; FLUID ; PH|
Background: Prostatitis is associated with a characteristic increase in prostatic fluid pH; however, the underlying mechanism and its physiological significance have not been elucidated.
Methodology/Principal Findings: In this study a primary culture of rat prostatic epithelial cells and a rat prostatitis model were used. Here we reported the involvement of CFTR, a cAMP-activated anion channel conducting both Cl(-) and O(3)(-), in mediating prostate HCO(3)(-) secretion and its possible role in bacterial killing. Upon Escherichia coli (E coli)-LPS challenge, the expression of CFTR and carbonic anhydrase II (CA II), along with several pro-inflammatory cytokines was up-regulated in the primary culture of rat prostate epithelial cells. Inhibiting CFTR function in vitro or in vivo resulted in reduced bacterial killing by prostate epithelial cells or the prostate. High HCO(3)(-) content (>50 mM), rather than alkaline pH, was found to be responsible for bacterial killing. The direct action of HCO(3)(-) on bacterial killing was confirmed by its ability to increase cAMP production and suppress bacterial initiation factors in E coli. The relevance of the CFTR-mediated HCO(3)(-) secretion in humans was demonstrated by the upregulated expression of CFTR and CAII in human prostatitis tissues.
Conclusions/Significance: The CFTR and its mediated HCO(3)(-) secretion may be up-regulated in prostatitis as a host defense mechanism.
|资助机构||Chinese University of Hong Kong|
|作者单位||1.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Epithelial Cell Biol Res Ctr, Hong Kong, Hong Kong, Peoples R China|
2.Sichuan Univ, W China Univ Hosp 2, Chinese Univ Hong Kong Joint Lab Reprod Med, Hong Kong, Hong Kong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod & Genet, Shenzhen, Peoples R China
|Xie, Chen,Tang, Xiaoxiao,Xu, Wenming,et al. A Host Defense Mechanism Involving CFTR-Mediated Bicarbonate Secretion in Bacterial Prostatitis[J]. PLOS ONE,2010,5(12).|
|APA||Xie, Chen,Tang, Xiaoxiao,Xu, Wenming,Diao, Ruiying,Cai, Zhiming,&Chan, Hsiao Chang.(2010).A Host Defense Mechanism Involving CFTR-Mediated Bicarbonate Secretion in Bacterial Prostatitis.PLOS ONE,5(12).|
|MLA||Xie, Chen,et al."A Host Defense Mechanism Involving CFTR-Mediated Bicarbonate Secretion in Bacterial Prostatitis".PLOS ONE 5.12(2010).|