北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 血液内科  > 期刊论文
学科主题: 临床医学
题名:
B7-H3 silencing by RNAi inhibits tumor progression and enhances chemosensitivity in U937 cells
作者: Zhang, Wei; Wang, Jing; Wang, Yanfang; Dong, Fei; Zhu, Mingxia; Wan, Wenli; Li, Haishen; Wu, Feifei; Yan, Xinxing; Ke, Xiaoyan1,2
关键词: B7-H3 ; acute monocytic leukemia ; cancer gene therapy ; chemosensitivity
刊名: ONCOTARGETS AND THERAPY
发表日期: 2015
DOI: 10.2147/OTT.S85272
卷: 8, 页:1721-1733
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Oncology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Oncology
关键词[WOS]: ACUTE MYELOID-LEUKEMIA ; COSTIMULATORY MOLECULE ; MONOCYTIC LEUKEMIA ; PANCREATIC-CANCER ; INDUCED APOPTOSIS ; LUNG-CANCER ; EXPRESSION ; OVEREXPRESSION ; SENSITIVITY ; METASTASIS
英文摘要:

Background: The role of B7-H3 in acute monocytic leukemia U937 cells has not been thoroughly investigated.

Materials and methods: B7-H3 knockdown in the U937 cell line was performed using small hairpin (sh)RNA lentivirus transduction. The effects on cell proliferation, cycle, migration, and invasion were investigated by Cell Counting Kit-8 assay, methyl cellulose colony-forming assay, propidium iodide staining, and Transwell assays in vitro. Changes in cell growth inhibition and apoptosis, when combined with chemotherapy drugs, were determined using the Cell Counting Kit-8 and Annexin V-FITC/PI assays. U937 xenograft models were used to assess the effects of B7-H3 on tumorigenicity and the therapeutic effect of B7-H3 knockdown in combination with chemotherapy drugs in vivo.

Results: Downregulation of B7-H3 significantly decreased U937 cell growth and colony-forming ability. The mean inhibition rate of tumor growth with B7-H3 knockdown was 59.4%, and the expression of both Ki-67 and PCNA in xenografts was significantly reduced. After B7-H3 silencing, the U937 cell cycle was arrested at the G0/G1 phase. The cell migration rate of B7-H3 knockdown cells was reduced more than fivefold, and invasion capacity decreased by 86.7%. B7-H3 RNAi profoundly increased the antitumor effect of chemotherapy in vitro and in vivo. On day 19, inhibition rates of tumor growth in B7-H3 shRNA combined with idarubicin, cytarabine, and idarubicin plus cytarabine were 70.5%, 80.0%, and 90.0%, respectively (P=0.006, P=0.004, and P=0.016, respectively).

Conclusion: B7-H3 may promote U937 cell progression, and shRNA targeting B7-H3 significantly enhances sensitivity to chemotherapeutic drugs. These results may provide new insight into the function of B7-H3 and a promising therapeutic approach targeting B7-H3 in acute monocytic leukemia.

语种: 英语
所属项目编号: 81172245
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000357878800006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54207
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Lymphoma Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Wei,Wang, Jing,Wang, Yanfang,et al. B7-H3 silencing by RNAi inhibits tumor progression and enhances chemosensitivity in U937 cells[J]. ONCOTARGETS AND THERAPY,2015,8:1721-1733.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Wei]'s Articles
[Wang, Jing]'s Articles
[Wang, Yanfang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Wei]‘s Articles
[Wang, Jing]‘s Articles
[Wang, Yanfang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace