|Decreased FOXD3 Expression Is Associated with Poor Prognosis in Patients with High-Grade Gliomas|
|Du, Wei1; Pang, Changhe1; Wang, Dongliang2; Zhang, Qingjun2; Xue, Yake1; Jiao, Hongliang1; Zhan, Lei3; Ma, Qian4; Wei, Xinting1|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||CHRONIC LYMPHOCYTIC-LEUKEMIA ; TRANSCRIPTION FACTOR FOXD3 ; GASTRIC CARCINOGENESIS ; TUMOR-SUPPRESSOR ; MELANOMA-CELLS ; MURINE MODEL ; CANCER ; PROGRESSION ; REQUIREMENT ; DISEASE|
The transcription factor forkhead box D3 (FOXD3) plays important roles in the development of neural crest and has been shown to suppress the development of various cancers. However, the expression and its potential biological roles of FOXD3 in high-grade gliomas (HGGs) remain unknown.
The mRNA and protein expression levels of FOXD3 were examined using real-time quantitative PCR and western blotting in 23 HGG and 13 normal brain samples, respectively. Immunohistochemistry was used to validate the expression FOXD3 protein in 184 HGG cases. The association between FOXD3 expression and the prognosis of HGG patients were analyzed using Kaplan-Meier survival curves and Cox proportional hazards regression models. In addition, we further examined the effects of FOXD3 on the proliferation and serum starvation-induced apoptosis of glioma cells.
In comparison to normal brain tissues, FOXD3 expression was significantly decreased in HGG tissues at both mRNA and protein levels. Immunohistochemistry further validated the expression of FOXD3 in HGG tissues. Moreover, low FOXD3 expression was significantly associated with poor prognosis in HGG patients. Depletion of FOXD3 expression promoted glioma cell proliferation and inhibited serum starvation-induced apoptosis, whereas overexpression of FOXD3 inhibited glioma cell proliferation and promoted serum starvation-induced apoptosis.
Our results indicated that FOXD3 might serve as an independent prognostic biomarker and a potential therapeutic target for HGGs, which warrant further investigation.
|资助机构||First Affiliated Hospital of Zhengzhou University|
|作者单位||1.Zhengzhou Univ, Affiliated Hosp 1, Dept Neurosurg, Zhengzhou 450052, Peoples R China|
2.Peking Univ, Peoples Hosp, Dept Neurosurg, Beijing 100044, Peoples R China
3.Harbin Med Univ, Affiliated Hosp 2, Dept Gastroenterol, Harbin 150086, Peoples R China
4.Zhengzhou Univ, Affiliated Hosp 1, Prenatal Diag Ctr, Dept Gynecol & Obstet, Zhengzhou 450052, Peoples R China
|Du, Wei,Pang, Changhe,Wang, Dongliang,et al. Decreased FOXD3 Expression Is Associated with Poor Prognosis in Patients with High-Grade Gliomas[J]. PLOS ONE,2015,10(5).|
|APA||Du, Wei.,Pang, Changhe.,Wang, Dongliang.,Zhang, Qingjun.,Xue, Yake.,...&Wei, Xinting.(2015).Decreased FOXD3 Expression Is Associated with Poor Prognosis in Patients with High-Grade Gliomas.PLOS ONE,10(5).|
|MLA||Du, Wei,et al."Decreased FOXD3 Expression Is Associated with Poor Prognosis in Patients with High-Grade Gliomas".PLOS ONE 10.5(2015).|