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Consistency Between Treponema pallidum Particle Agglutination Assay and Architect Chemiluminescent Microparticle Immunoassay and Characterization of Inconsistent Samples
Li Zhiyan; Wang Meiling; Liu Ping; Dong Jinhua; Yan Zhenlin; Feng Zhenru
关键词Treponema Pallidum Syphilis Serological Test Screening
刊名JOURNAL OF CLINICAL LABORATORY ANALYSIS
2015-07-01
DOI10.1002/jcla.21765
29期:4页:281-284
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]HUMAN-IMMUNODEFICIENCY-VIRUS ; SYPHILIS ; TESTS ; INFECTION ; CHINA
英文摘要

BackgroundTreponema pallidum particle agglutination assay (TPPA) has been shown to be highly sensitive and specific at detecting treponemal antibodies and is still used as a confirmatory method in many laboratories, in China. In clinical practice, we found that a significant number of TPPA-negative sera were identified when TPPA was used as a confirmatory assay of Architect chemiluminescent microparticle immunoassay (CMIA) screening-reactive sera.

AimsTo investigate the consistency between Architect CMIA and TPPA, and analyzed the characterization of TPPA-negative sera following Screening by Architect CMIA.

MethodsAccording to the laboratory syphilis confirmatory testing protocol, a total of 4870 sera were initially tested by Architect CMIA and ELISA, and then the samples which shown positive results were tested by TPPA and rapid plasma reagin tests (RPR). Further analysis using Euroimmun dot-immunoblot (dot-IBT) assay was performed to the CMIA positive and TPPA negative samples.

ResultsIn our cohort, we found that the positive rate of CMIA was 3.1% (149/4870). One hundred and twelve of 112 (75.2%) CMIA-positive sera were TPPA reactive, while 37 (24.8%) sera which were reactive in CMIA were nonreactive by TPPA. Dot-IBT testing was performed on these 37 sera: 8 (21.6%) were dot-IBT positive, 11 (29.7%) were indeterminate and 18 (48.6%) negative.

DiscussionIn this study, we observed that 18 CMIA-positive sera were false positives confirmed by dot-IBT. But, given the relatively high levels of early syphilis, we consider a small increase in the number of confirmatory tests is worthwhile if we can increase the detection of primary syphilis by 20%. We also found that significant numbers (8/37) of CMIA-positive and TPPA-negative sera were shown by further dot-IBT testing to be positive. The reason why certain sera are negative by TPPA but reactive by CMIA and other syphilis confirmatory assays is not clear, and these initial findings should be further explored.

ConclusionThe Architect CMIA is a highly sensitive screening assay for detecting syphilis but it is significantly less specific. Further analysis by TPPA is recommended to confirm the results. We would highlight the fact that in repeatedly screened populations discrepancies between treponemal CMIA and TPPA results are quite prevalent. This seems to be a function of very low levels of syphilis-specific antibodies. Confirmation by immunoblot assay may be useful.

语种英语
WOS记录号WOS:000358250300006
项目编号2012BAH24F00
资助机构National Key Technology RD Program
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54228
专题北京大学第一临床医学院
北京大学第一临床医学院_检验科
作者单位Peking Univ, Dept Clin Lab, Hosp 1, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Li Zhiyan,Wang Meiling,Liu Ping,et al. Consistency Between Treponema pallidum Particle Agglutination Assay and Architect Chemiluminescent Microparticle Immunoassay and Characterization of Inconsistent Samples[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2015,29(4):281-284.
APA Li Zhiyan,Wang Meiling,Liu Ping,Dong Jinhua,Yan Zhenlin,&Feng Zhenru.(2015).Consistency Between Treponema pallidum Particle Agglutination Assay and Architect Chemiluminescent Microparticle Immunoassay and Characterization of Inconsistent Samples.JOURNAL OF CLINICAL LABORATORY ANALYSIS,29(4),281-284.
MLA Li Zhiyan,et al."Consistency Between Treponema pallidum Particle Agglutination Assay and Architect Chemiluminescent Microparticle Immunoassay and Characterization of Inconsistent Samples".JOURNAL OF CLINICAL LABORATORY ANALYSIS 29.4(2015):281-284.
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