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学科主题: 药学
题名:
Structural characterization of metabolites of salvianolic acid B from Salvia miltiorrhiza in normal and antibiotic-treated rats by liquid chromatography-mass spectrometry
作者: Xu, Man; Guo, Hui; Han, Jian; Sun, Shi-Feng; Liu, Ai-Hua; Wang, Bao-Rong; Ma, Xiao-Chi; Liu, Peng; Qiao, Xue; Zhang, Zi-Chuan; Guo, De-An
关键词: salvianolic acid B ; in vivo metabolites ; LC-MS" ; intestinal bacterial ; antibiotic-treated rats
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-10-15
DOI: 10.1016/j.jchromb.2007.08.032
卷: 858, 期:1-2, 页:184-198
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: MAGNESIUM LITHOSPERMATE-B ; SMOOTH-MUSCLE-CELLS ; BEAGLE DOG SERUM ; PHENOLIC-ACIDS ; HUMAN URINE ; IN-VITRO ; DIETARY POLYPHENOLS ; ROSMARINIC ACID ; ORGANIC-ACIDS ; HPLC-DAD
英文摘要:

This study was conducted to compare the in vivo metabolites of salvianolic acid B (Sal B) between normal rats and antibiotic-treated rats and to clarify the role of intestinal bacteria on the absorption, metabolism and excretion of Sal B. A valid method using LC-MS" analysis was established for identification of rat biliary and fecal metabolites. And isolation of normal rat urinary metabolites by repeated column chromatography was applied in this study. Four biliary metabolites and five fecal metabolites in normal rats were identified on the basis of their MS′ fragmentation patterns. Meanwhile, two normal rat urinary metabolites were firstly identified on the basis of their NMR and MS data. In contrast, no metabolites were detected in antibiotic-treated rat urine and bile, while the prototype of Sal B was found in antibiotic-treated rat feces. The differences of in vivo metabolites between normal rats and antibiotic-treated rats were proposed for the first time. Furthermore, it was indicated that the intestinal bacteria showed an important role on the absorption, metabolism and excretion of Sal B. This investigation provided scientific evidence to infer the active principles responsible for the pharmacological effects of Sal B. (C) 2007 Published by Elsevier B.V.

语种: 英语
WOS记录号: WOS:000250608200024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54234
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Ctr Hlth Sci, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Xu, Man,Guo, Hui,Han, Jian,et al. Structural characterization of metabolites of salvianolic acid B from Salvia miltiorrhiza in normal and antibiotic-treated rats by liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,858(1-2):184-198.
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