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学科主题: 口腔医学
题名:
Effect of Nicotine and Porphyromonas gingivalis Lipopolysaccharide on Endothelial Cells In Vitro
作者: An, Na1; Andrukhov, Oleh2; Tang, Yan2,3; Falkensammer, Frank4; Bantleon, Hans-Peter4; Ouyang, Xiangying5; Rausch-Fan, Xiaohui2,4
刊名: PLOS ONE
发表日期: 2014-05-12
DOI: 10.1371/journal.pone.0096942
卷: 9, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ADHESION MOLECULE EXPRESSION ; FIMBRIA-DEPENDENT ACTIVATION ; PERIODONTAL-DISEASE ; LEUKOCYTE ADHESION ; CIGARETTE-SMOKING ; ATHEROSCLEROSIS ; MIGRATION ; SMOKERS ; INTERLEUKIN-8 ; INFLAMMATION
英文摘要:

Smoking is considered a significant risk factor for both periodontal disease and cardiovascular disease (CVD). Endothelial cells play an important role in the progression of both diseases. In the present study, we investigated in vitro the impact of nicotine on functional properties of human umbilical vein endothelial cells (HUVECs) stimulated with lipopolysaccharide (LPS) of periodontal pathogen Porphyromonas gingivalis. HUVECs were stimulated with different concentrations of nicotine (10 mu M-10 mM) and/or P. gingivalis LPS. Expression levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, E-selectin, monocyte chemoattractant protein 1, and interleukin-8 were measured on both gene and protein levels. Cell proliferation/viability, apoptosis, and migration were also investigated. Nicotine at a concentration of 10 mM significantly decreased P. gingivalis LPS-induced expression of all investigated proteins after 4 h stimulation, while lower nicotine concentrations had no significant effect on protein expression with or without P. gingivalis LPS. Proliferation/viability of HUVECs was also significantly inhibited by 10-mM nicotine but not by lower concentrations. Migration of HUVECs was significantly decreased by nicotine at concentrations of 1-10 mM. Nicotine at a concentration similar to that observed in the serum of smokers had no significant effect on the functional properties of HUVECs. However, high concentrations of nicotine, similar to that observed in the oral cavity of smokers, inhibited the inflammatory response of HUVECs. This effect of nicotine might be associated with decreased gingival bleeding indices in smoking periodontitis patients.

语种: 英语
所属项目编号: 1019
项目资助者: Ministry of Science and Technology of China under contract International Science &amp ; Technology Cooperation Program Foundation
WOS记录号: WOS:000336653300052
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54241
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Gen Dent 2, Beijing 100871, Peoples R China
2.Med Univ Vienna, Bernhard Gottlieb Sch Dent, Div Oral Biol, Vienna, Austria
3.Capital Med Univ, Xuanwu Hosp, Dept Stomatol, Beijing, Peoples R China
4.Med Univ Vienna, Bernhard Gottlieb Sch Dent, Div Orthodont, Vienna, Austria
5.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China

Recommended Citation:
An, Na,Andrukhov, Oleh,Tang, Yan,et al. Effect of Nicotine and Porphyromonas gingivalis Lipopolysaccharide on Endothelial Cells In Vitro[J]. PLOS ONE,2014,9(5).
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