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学科主题: 临床医学
题名:
Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads
作者: Zhang, WJ; Lin, ZX; Hu, Y
关键词: latex bead ; N-cadherin ; adherens junction ; adhesion molecular complex
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2000-08-01
卷: 45, 期:16, 页:1504-1509
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要:

Cadherin receptors mediate cell-cell adhesion, signal transduction and assembly of cytoskeletons. How a single transmembrane molecule Cadherin can be involved in multiple functions through modulating its binding activities with many membrane adhesion molecules and cytoskeletal components is an unanswered question which can be elucidated by clues from bead experiments. Human lung cells expressing N-Cadherin were examined. After co-incubation with anti-N-Cadherin monoclonal antibody coated beads, cell surface clustering of N-Cadherin was induced. Immunofluorescent detection demonstrated that in addition to Cadherin, beta-Catenin, alpha-Catenin, alpha-Actinin and Actin fluorescence also aggregated respectively at the membrane site of bead attachment. Myosin heavy chain (MHC), another major component of Actin cytoskeleton, did not aggregate at the membrane site of bead attachment. Adhesion unrelated protein Con A and polylysine conjugated beads did not induce the clustering of adhesion molecules. It is indicated that the Cadherin/Catenins/alpha-Actinin/Actin complex is formed at Cadherin mediated cell adherens junction; occupancy and cell surface clustering of Cadherin is crucial for the formation of Cadherin adhesion protein complexes.

语种: 英语
WOS记录号: WOS:000089247600015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54252
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: Beijing Med Univ, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, WJ,Lin, ZX,Hu, Y. Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads[J]. CHINESE SCIENCE BULLETIN,2000,45(16):1504-1509.
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