IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads
Zhang, WJ; Lin, ZX; Hu, Y
关键词Latex Bead N-cadherin Adherens Junction Adhesion Molecular Complex
刊名CHINESE SCIENCE BULLETIN
2000-08-01
45期:16页:1504-1509
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
英文摘要

Cadherin receptors mediate cell-cell adhesion, signal transduction and assembly of cytoskeletons. How a single transmembrane molecule Cadherin can be involved in multiple functions through modulating its binding activities with many membrane adhesion molecules and cytoskeletal components is an unanswered question which can be elucidated by clues from bead experiments. Human lung cells expressing N-Cadherin were examined. After co-incubation with anti-N-Cadherin monoclonal antibody coated beads, cell surface clustering of N-Cadherin was induced. Immunofluorescent detection demonstrated that in addition to Cadherin, beta-Catenin, alpha-Catenin, alpha-Actinin and Actin fluorescence also aggregated respectively at the membrane site of bead attachment. Myosin heavy chain (MHC), another major component of Actin cytoskeleton, did not aggregate at the membrane site of bead attachment. Adhesion unrelated protein Con A and polylysine conjugated beads did not induce the clustering of adhesion molecules. It is indicated that the Cadherin/Catenins/alpha-Actinin/Actin complex is formed at Cadherin mediated cell adherens junction; occupancy and cell surface clustering of Cadherin is crucial for the formation of Cadherin adhesion protein complexes.

语种英语
WOS记录号WOS:000089247600015
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54252
专题北京大学临床肿瘤学院
作者单位Beijing Med Univ, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China
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GB/T 7714
Zhang, WJ,Lin, ZX,Hu, Y. Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads[J]. CHINESE SCIENCE BULLETIN,2000,45(16):1504-1509.
APA Zhang, WJ,Lin, ZX,&Hu, Y.(2000).Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads.CHINESE SCIENCE BULLETIN,45(16),1504-1509.
MLA Zhang, WJ,et al."Cell surface clustering of Cadherin adhesion complex induced by antibody coated beads".CHINESE SCIENCE BULLETIN 45.16(2000):1504-1509.
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