学科主题基础医学
In vitro neuronal differentiation of cultured human embryonic germ cells
Pan, YQ; Chen, X; Wang, SL; Yang, SF; Bai, XW; Chi, XC; Li, K; Liu, B; Li, LS
关键词human embryonic germ cells human embryonic stern cells induction neuronal differentiation primordial germ cells cyclic AMP forskolin RA
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2005-02-11
DOI10.1016/j.bbrc.2004.11.168
327期:2页:548-556
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PLURIPOTENT STEM-CELLS ; HUMAN BLASTOCYSTS ; PROGENITORS ; DERIVATION ; MARKERS ; LINES ; CAMP
英文摘要

Human embryonic germ (hEG) cells, which have been advanced as one of the most important sources of pluripotent stem cells [the other one being human embryonic stem cells]. can be propagated in vitro indefinitely in the primitive undifferentiated state while being capable of developing into all three gem layer derivatives. hence have become anticipated developing novel strategies of tissue regeneration and transplantation in the treatment of degenerative diseases. In the experiments here, we derived hEG cells from cultured human primordial germ cells (PGCs) of 6- to 9-week-post-fertilization embryos. They satisfied the criteria previously used to define hEG cells, including the expression of markers characteristic of pluripotent cells-abundant alkaline phosphatase (AP) activity, stage specific embryonic antigen (SSEA)-1(+), SSEA-3(-), SSEA-4(+). TRA-1-60(+). TRA-1-81(+), Oct-4(+), and hTERT(+), the retention of normal karyotypes, and possessing pluripotency by forming embryoid bodies (EBs) in vitro. Furthermore, these derived cells tended to neurally differentiate in vitro, especially under high-density culture conditions. We Successfully isolated neural progenitor cells from differentiating hEG cultures and about 10% cells induced by 2 muM all-trans-retinoic acid (RA) or 0.1 mM dibutyryl cyclic AMP (dbcAMP)/1 mM forskolin to mature neurons expressing microtubule-associated protein 2ab (MAP2ab), synaptophysin, beta-tubulin III, neuron-specific enolase (NSE), tyrosine hydroxylase (TH), but no glial fibrillary acid protein (GFAP) and choline acetyl transferase (ChAT). The data suggested that hEG cells may provide a potential source of cells for use in transplantation therapy for neurological degenerative diseases. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000226364000023
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54257
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Anat Histol & Embryol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Pan, YQ,Chen, X,Wang, SL,et al. In vitro neuronal differentiation of cultured human embryonic germ cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,327(2):548-556.
APA Pan, YQ.,Chen, X.,Wang, SL.,Yang, SF.,Bai, XW.,...&Li, LS.(2005).In vitro neuronal differentiation of cultured human embryonic germ cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,327(2),548-556.
MLA Pan, YQ,et al."In vitro neuronal differentiation of cultured human embryonic germ cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 327.2(2005):548-556.
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