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学科主题: 基础医学
题名:
In vitro neuronal differentiation of cultured human embryonic germ cells
作者: Pan, YQ; Chen, X; Wang, SL; Yang, SF; Bai, XW; Chi, XC; Li, K; Liu, B; Li, LS
关键词: human embryonic germ cells ; human embryonic stern cells ; induction ; neuronal differentiation ; primordial germ cells ; cyclic AMP ; forskolin ; RA
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-02-11
DOI: 10.1016/j.bbrc.2004.11.168
卷: 327, 期:2, 页:548-556
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PLURIPOTENT STEM-CELLS ; HUMAN BLASTOCYSTS ; PROGENITORS ; DERIVATION ; MARKERS ; LINES ; CAMP
英文摘要:

Human embryonic germ (hEG) cells, which have been advanced as one of the most important sources of pluripotent stem cells [the other one being human embryonic stem cells]. can be propagated in vitro indefinitely in the primitive undifferentiated state while being capable of developing into all three gem layer derivatives. hence have become anticipated developing novel strategies of tissue regeneration and transplantation in the treatment of degenerative diseases. In the experiments here, we derived hEG cells from cultured human primordial germ cells (PGCs) of 6- to 9-week-post-fertilization embryos. They satisfied the criteria previously used to define hEG cells, including the expression of markers characteristic of pluripotent cells-abundant alkaline phosphatase (AP) activity, stage specific embryonic antigen (SSEA)-1(+), SSEA-3(-), SSEA-4(+). TRA-1-60(+). TRA-1-81(+), Oct-4(+), and hTERT(+), the retention of normal karyotypes, and possessing pluripotency by forming embryoid bodies (EBs) in vitro. Furthermore, these derived cells tended to neurally differentiate in vitro, especially under high-density culture conditions. We Successfully isolated neural progenitor cells from differentiating hEG cultures and about 10% cells induced by 2 muM all-trans-retinoic acid (RA) or 0.1 mM dibutyryl cyclic AMP (dbcAMP)/1 mM forskolin to mature neurons expressing microtubule-associated protein 2ab (MAP2ab), synaptophysin, beta-tubulin III, neuron-specific enolase (NSE), tyrosine hydroxylase (TH), but no glial fibrillary acid protein (GFAP) and choline acetyl transferase (ChAT). The data suggested that hEG cells may provide a potential source of cells for use in transplantation therapy for neurological degenerative diseases. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000226364000023
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54257
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Anat Histol & Embryol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Pan, YQ,Chen, X,Wang, SL,et al. In vitro neuronal differentiation of cultured human embryonic germ cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,327(2):548-556.
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