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1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway
Chen, Shanwen1; Zhu, Jing1; Chen, Guowei1; Zuo, Shuai1; Zhang, Junling1; Chen, Ziyi1; Wang, Xin1; Li, Junxia2; Liu, Yucun1; Wang, Pengyuan1
关键词Tnp-alpha Tight Junction (Tj) 1 Trans-epithelial Electrical Resistance (Teer) 25(Oh)2d3 Mlck
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2015-05-08
DOI10.1016/j.bbrc.2015.03.125
460期:3页:873-878
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]LIGHT-CHAIN KINASE ; TIGHT JUNCTION PERMEABILITY ; INTERFERON-GAMMA ; IN-VITRO ; DISRUPTION ; ACTIVATION ; EXPRESSION ; DYSFUNCTION ; MECHANISMS ; INCREASE
英文摘要

Substantial studies have demonstrated the protective effect of 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) on intestinal barrier function, but the mechanisms are not fully illustrated. In this study, the effect of 1,25(OH)2D3 on TNF-alpha induced barrier dysfunction was further investigated in Caco-2 cell monolayers. The barrier function of Caco-2 monolayers was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC-Dextran 40,000 Da (FD-40) trans-membrane flux. ZO-1 and Occludin were chosen as markers of the localization of tight junction (TJ) proteins for immunofluorescence. The expression of MLCK and phosphorylation level of myosin light chain (MLC) were measured by immunoblotting. The activation of NF-kB p65 was analyzed by EMSA and immunofluorescence. The results suggest that 1,25(OH)2D3 preserves intestinal epithelial barrier function from TNF-a. induced injury via suppression of NF-kB p65 mediated activation of MLCK-P-MLC signaling pathway. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000359885300062
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54265
专题北京大学第一临床医学院
北京大学第一临床医学院_普通外科
北京大学第一临床医学院_消化科
作者单位1.Peking Univ, Hosp 1, Div Gen Surg, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Div Gastroenterol, Beijing 100034, Peoples R China
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Chen, Shanwen,Zhu, Jing,Chen, Guowei,et al. 1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,460(3):873-878.
APA Chen, Shanwen.,Zhu, Jing.,Chen, Guowei.,Zuo, Shuai.,Zhang, Junling.,...&Wang, Pengyuan.(2015).1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,460(3),873-878.
MLA Chen, Shanwen,et al."1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 460.3(2015):873-878.
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