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学科主题: 临床医学
题名:
1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway
作者: Chen, Shanwen1; Zhu, Jing1; Chen, Guowei1; Zuo, Shuai1; Zhang, Junling1; Chen, Ziyi1; Wang, Xin1; Li, Junxia2; Liu, Yucun1; Wang, Pengyuan1
关键词: TNP-alpha ; 1,25(OH)2D3 ; Tight junction (TJ) ; Trans-epithelial electrical resistance (TEER) ; MLCK
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2015-05-08
DOI: 10.1016/j.bbrc.2015.03.125
卷: 460, 期:3, 页:873-878
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: LIGHT-CHAIN KINASE ; TIGHT JUNCTION PERMEABILITY ; INTERFERON-GAMMA ; IN-VITRO ; DISRUPTION ; ACTIVATION ; EXPRESSION ; DYSFUNCTION ; MECHANISMS ; INCREASE
英文摘要:

Substantial studies have demonstrated the protective effect of 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) on intestinal barrier function, but the mechanisms are not fully illustrated. In this study, the effect of 1,25(OH)2D3 on TNF-alpha induced barrier dysfunction was further investigated in Caco-2 cell monolayers. The barrier function of Caco-2 monolayers was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC-Dextran 40,000 Da (FD-40) trans-membrane flux. ZO-1 and Occludin were chosen as markers of the localization of tight junction (TJ) proteins for immunofluorescence. The expression of MLCK and phosphorylation level of myosin light chain (MLC) were measured by immunoblotting. The activation of NF-kB p65 was analyzed by EMSA and immunofluorescence. The results suggest that 1,25(OH)2D3 preserves intestinal epithelial barrier function from TNF-a. induced injury via suppression of NF-kB p65 mediated activation of MLCK-P-MLC signaling pathway. (C) 2015 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000359885300062
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54265
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Div Gen Surg, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Div Gastroenterol, Beijing 100034, Peoples R China

Recommended Citation:
Chen, Shanwen,Zhu, Jing,Chen, Guowei,et al. 1,25-Dihydroxyvitamin D3 preserves intestinal epithelial barrier function from TNF-alpha induced injury via suppression of NF-kB p65 mediated MLCK-P-MLC signaling pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,460(3):873-878.
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