学科主题基础医学
Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase
Hao, Hui-Feng1,2; Liu, Li-Mei3; Liu, Yu-Ying1; Liu, Juan3; Yan, Li1; Pan, Chun-Shui1; Wang, Ming-Xia1; Wang, Chuan-She1,2; Fan, Jing-Yu1; Gao, Yuan-Sheng3; Han, Jing-Yan1,2
关键词Endothelin 1 Vasodilation Rhoa-rock L-vdcc
刊名JOURNAL OF ETHNOPHARMACOLOGY
2014-08-08
DOI10.1016/j.jep.2014.04.050
155期:1页:147-153
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
资助者Tianjin Tasly Group, Tianjin, China ; National Natural Science Foundation of China ; Tianjin Tasly Group, Tianjin, China ; National Natural Science Foundation of China
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]VASCULAR SMOOTH-MUSCLE ; IN-VITRO ; SIGNALING PATHWAY ; MESENTERIC-ARTERY ; CA2+ CHANNELS ; ACTIVATION ; HYPERTENSION ; RATS ; PATHOPHYSIOLOGY ; MECHANISMS
英文摘要

Ethnopharmacological relevance: Rhynchophylline (Rhy) is a major ingredient of Uncaria rhynchophylla (UR) used to reduce blood pressure and ameliorate brain ailments. This study was to examine the role of Rho kinase (ROCK) in the inhibition of Rhy on contraction of cerebral arterioles caused by endothelin 1 (ET-1).

Materials and methods: Cerebral arterioles of male Wistar rats were constricted with ET-1 for 10 min followed by perfusion of Rhy for 20 min. Changes in the diameters of the arterioles were recorded. The effects of Rhy on contraction of middle cerebral arteries (MCAs) were determined by a Multi-Myograph. Western blotting and immunofluorescent staining were used to examine the effects of Rhy on RhoA translocation and myosin phosphatase target subunit 1 (MYPT1) phosphorylation.

Results: In vivo, Rhy (30-300 mu M) relaxed cerebral arterioles constricted with ET-1 dose-dependently. In vitro, Rhy at lower concentrations (1-100 mu M) caused relaxation of rat MCAs constricted with KCl and Bay-K8644 (an agonist of L-type voltage-dependent calcium channels (L-VDCCs)). Rhy at higher concentrations (> 100 mu M) caused relaxation of rat MCAs constricted with ET-1, which was inhibited by Y27632, a ROCK′s inhibitor. Weftern blotting of rat aortas showed that Rhy inhibited RhoA translocation and MYPT1 phosphorylation. Immunofluorescent staining of MCAs confirmed that phosphorylation of MYPT1 caused by ET-1 was inhibited by Rhy.

Conclusions: These results demonstrate that Rhy is a potent inhibitor of contraction of cerebral arteries caused by ET-1 in vivo and in vitro. The effect of Rhy was in part mediated by inhibiting RhoA-ROCK signaling. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
所属项目编号2010ZX09401-406 ; 81270341
资助者Tianjin Tasly Group, Tianjin, China ; National Natural Science Foundation of China ; Tianjin Tasly Group, Tianjin, China ; National Natural Science Foundation of China
WOS记录号WOS:000340854000013
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54276
Collection北京大学基础医学院_北大医学部天士力微循环研究中心
作者单位1.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Hao, Hui-Feng,Liu, Li-Mei,Liu, Yu-Ying,et al. Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,155(1):147-153.
APA Hao, Hui-Feng.,Liu, Li-Mei.,Liu, Yu-Ying.,Liu, Juan.,Yan, Li.,...&Han, Jing-Yan.(2014).Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase.JOURNAL OF ETHNOPHARMACOLOGY,155(1),147-153.
MLA Hao, Hui-Feng,et al."Inhibitory effect of rhynchophylline on contraction of cerebral arterioles to endothelin 1: Role of rho kinase".JOURNAL OF ETHNOPHARMACOLOGY 155.1(2014):147-153.
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