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学科主题基础医学
Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity
Zhu, Haihao1,2; Yang, Yanrui1,2; Zhang, Hua3; Han, Yan1,2; Li, Yafang1,2; Zhang, Ying1,2; Yin, Dongmin1,2; He, Qihua4; Zhao, Zhiqi3; Blumberg, Peter M.5; Han, Jisheng1,2; Wang, Yun1,2
关键词Protein Kinase D1 (pkD1) Transient Receptor Potential V1 (trpV1) Inflammatory Heat Hypersensitivity Gene Delivery Intrathecal Injection
刊名PAIN
2008-07-31
DOI10.1016/j.pain.2007.10.025
137期:3页:574-588
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Anesthesiology ; Clinical Neurology ; Neurosciences
研究领域[WOS]Anesthesiology ; Neurosciences & Neurology
关键词[WOS]DORSAL-ROOT GANGLIA ; RAT SPINAL-CORD ; NF-KAPPA-B ; PERIPHERAL INFLAMMATION ; PAIN HYPERSENSITIVITY ; VANILLOID RECEPTOR-1 ; CAPSAICIN RECEPTOR ; NEUROPATHIC PAIN ; IN-VIVO ; C-MU
英文摘要

In previous studies we demonstrated that protein kinase D1 (PKD1/PKC mu) Could directly phosphorylate the transient receptor potential V1 (TRPV1) at its N-terminal region and enhance the function of TRPV1 in CHO cells stably transfected with TRPV1. In the Current study we assessed the involvement of PKD1 in pain modulation and explored the possible interaction between PKD1 and TRPV1 in rat inflammatory heat hypersensitivity. PKD1 was translocated to cytoplasmic membrane fraction and was trans-phosphorylated only in membrane fraction but not in cytoplasmic fraction of dorsal root ganglia (DRG) at 2 and 6 h after Complete Freund′s Adjuvant (CFA) treatment. Pre i.t. injection of PKD1 antisense for 4 d or post-i.t. injection for 4 d both alleviated CFA-induced thermal hypersensitivity. Likewise. overexpression of PKD1 in DRG significantly enhanced. while dominant negative PKD1 (DN-PKD1) partly attenuated. heat hypersensitivity. Both PKD1 and TRPV1 were translocated to the cytoplasmic membrane in DRG 6 h after CFA treatment and. at that time. PKID1 interacted with TRPV1 by co-immunoprecipitation in DRG. Electrophysiological measurements indicated that DRG with overexpression of PKID1 were more sensitive to low dose capsaicin than those expressing DN-PKD1. The average magnitude of the peak inward current evoked by capsaicin was greater in the DRG over-expressing PKD1 than in those expressing DN-PKD1. Furthermore. overexpressed PKD1 could up regulate. whereas PKD1 antisense could knock down TRPV1 content in DRG through posttranscriptional regulation manner. We concluded that PKD1 in DRG, through interaction with TRPV1. is involved in developing and maintaining inflammatory heat hypersensitivity. (C) 2007 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000258359500016
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/54284
专题北京大学基础医学院_神经生物学系
北京大学医学部管理机构_医学部
北京大学基础医学院
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Fudan Univ, Inst Neurobiol, Shanghai 200433, Peoples R China
3.Peking Univ, Dept Neurobiol, Key Lab Neurosci, Beijing 100083, Peoples R China
4.Peking Univ, Healthy Analyt Ctr, Beijing 100871, Peoples R China
5.NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA
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Zhu, Haihao,Yang, Yanrui,Zhang, Hua,et al. Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity[J]. PAIN,2008,137(3):574-588.
APA Zhu, Haihao.,Yang, Yanrui.,Zhang, Hua.,Han, Yan.,Li, Yafang.,...&Wang, Yun.(2008).Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity.PAIN,137(3),574-588.
MLA Zhu, Haihao,et al."Interaction between protein kinase D1 and transient receptor potential V1 in primary sensory neurons is involved in heat hypersensitivity".PAIN 137.3(2008):574-588.
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