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学科主题: 药学
题名:
Integrin-mediated signaling contributes to gadolinium-containing-particle-promoted cell survival and G(1) to S phase cell cycle transition by enhancing focal adhesion formation
作者: Li, Jin-Xia1,2; Fu, Li-Juan1; Yang, Xiao-Gai1; Wang, Kui1,2
关键词: Gadolinium ; Integrin ; Focal adhesion ; Nephrogenic systemic fibrosis ; Cell proliferation
刊名: JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
发表日期: 2012-03-01
DOI: 10.1007/s00775-011-0859-7
卷: 17, 期:3, 页:375-385
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: NEPHROGENIC SYSTEMIC FIBROSIS ; GROWTH-FACTOR RECEPTOR ; IMAGING CONTRAST AGENTS ; RARE-EARTH ELEMENTS ; DEPENDENT REGULATION ; EPITHELIAL-CELLS ; STRUCTURAL BASIS ; ACTIVATION ; PROLIFERATION ; FIBROBLASTS
英文摘要:

We previously reported that Gd-containing particles formed under physiological conditions act as active entities to enhance cell survival and promote S phase entry via activation of both mitogen-activated protein kinase/extracellular-signal-regulated protein kinase (ERK) and phosphatidylinositol 3-kinase/Akt signaling pathways. However, how they transduce the extracellular signal inside the cell remains unclear. The present study demonstrates that Gd-containing particles can alleviate serum-deprivation-induced cell death and promote G(1) to S phase cell cycle progression by enhancing cell adhesion to the extracellular matrix. As an indicator of adhesion, the vinculin distribution was detected by confocal laser scanning microscopy. The control cells exhibited fewer and less typical focal adhesions. After treatment with Gd-containing particles, a large number of vinculin-containing focal adhesions were maintained. In the presence of integrin antagonists, the percentage of S phase entry induced by Gd-containing particles was decreased and the enhancement of cell viability was also attenuated, along with a decrease in both cyclin D expression and ERK phosphorylation. In summary, the present results suggest that the integrin-mediated signaling pathway plays an important role in cell survival and G(1) to S phase transition promoted by Gd-containing particles by enhancing focal adhesion formation. The results presented here provide novel evidence to advance knowledge leading to further understanding of the mechanisms of both cell proliferation and cell survival promoted by Gd and may be helpful for developing effective measures to prevent or treat nephrogenic systemic fibrosis.

语种: 英语
所属项目编号: 20637010
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000301186000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54291
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Li, Jin-Xia,Fu, Li-Juan,Yang, Xiao-Gai,et al. Integrin-mediated signaling contributes to gadolinium-containing-particle-promoted cell survival and G(1) to S phase cell cycle transition by enhancing focal adhesion formation[J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY,2012,17(3):375-385.
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