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学科主题: 临床医学
题名:
Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells
作者: Yang, Yuejing1; Xin, Zhongcheng2; Chu, Jingjia3; Li, Na3; Sun, Tao3,4
关键词: CD83 ; Caveolin ; Dendritic cells ; Endocytosis ; Transplantation
刊名: CELL TRANSPLANTATION
发表日期: 2015
DOI: 10.3727/096368914X682116
卷: 24, 期:7, 页:1395-1404
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: SOLUBLE CD83 ; RENAL-TRANSPLANTATION ; ALLOGRAFT-REJECTION ; T-CELLS ; ENDOCYTOSIS ; DISEASE ; ACTIVATION ; PREVENTION ; MATURATION ; INDUCTION
英文摘要:

To become potent T-cell stimulators, DCs need to mature. Treatment with soluble CD83 (sCD83) induces immune tolerance and protects against transplant rejection by maintaining dendritic cells in an immature, tolerogenic state. Until now, the mechanism through which sCD83 keeps DCs immature has not been investigated. The internalizing pathway of CD83 was screened by Western blot, and the direct interactions between internalized proteins were verified through coimmunoprecipitation (co-IP) and transmission electron microscopy (TEM). CD83 plasma membrane levels were detected by Western blot using a plasma membrane protein extraction protocol. The changes in CD83 surface levels in DCs were detected by flow cytometry. Caveolin-1 function was detected in a kidney transplant model. In this study, we demonstrated that caveolin-1 could affect CD83 level during endocytosis in mouse DCs. Caveolin-1 coprecipitates with CD83, as demonstrated by co-IP analysis. TEM morphometric analysis of the entire CD83 distribution associated with internalized caveolin-1 demonstrated a significant interaction in cellular vesicles. sCD83 reduces endogenous CD83 plasma membrane levels, and caveolin-1 knockdown reverts CD83 levels in plasma membrane. sCD83 treatment decreases CD83 surface levels in DCs. siRNA to caveolin-1 in DCs inhibits this effect of sCD83. The effects of sCD83-treated DCs were proved in CD1 mice. Knocking down caveolin-1 in DCs obstructs the effects of sCD83 on kidney transplant. In conclusion, our data indicated that a caveolin-dependent endocytic pathway is involved in CD83 internalization in DCs and that caveolin-1 is involved in the activity of DCs.

语种: 英语
项目资助者: Chinese 5451 project of Henan Province Health Department
WOS记录号: WOS:000357760500015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54305
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Zhengzhou Univ, Affiliated Hosp 2, Zhengzhou 450052, Peoples R China
2.Univ Western Ontario, Western Sci Ctr, London, ON N6A 4G5, Canada
3.Peking Univ, Hosp 1, Dept Urol, Androl Ctr, Beijing 100871, Peoples R China
4.Univ Western Ontario, Lawson Hlth Res Inst, Crit Illness Res, London, ON N6A 4G5, Canada

Recommended Citation:
Yang, Yuejing,Xin, Zhongcheng,Chu, Jingjia,et al. Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells[J]. CELL TRANSPLANTATION,2015,24(7):1395-1404.
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