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学科主题临床医学
Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells
Yang, Yuejing1; Xin, Zhongcheng2; Chu, Jingjia3; Li, Na3; Sun, Tao3,4
关键词Cd83 Caveolin Dendritic Cells Endocytosis Transplantation
刊名CELL TRANSPLANTATION
2015
DOI10.3727/096368914X682116
24期:7页:1395-1404
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]Cell Biology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]SOLUBLE CD83 ; RENAL-TRANSPLANTATION ; ALLOGRAFT-REJECTION ; T-CELLS ; ENDOCYTOSIS ; DISEASE ; ACTIVATION ; PREVENTION ; MATURATION ; INDUCTION
英文摘要

To become potent T-cell stimulators, DCs need to mature. Treatment with soluble CD83 (sCD83) induces immune tolerance and protects against transplant rejection by maintaining dendritic cells in an immature, tolerogenic state. Until now, the mechanism through which sCD83 keeps DCs immature has not been investigated. The internalizing pathway of CD83 was screened by Western blot, and the direct interactions between internalized proteins were verified through coimmunoprecipitation (co-IP) and transmission electron microscopy (TEM). CD83 plasma membrane levels were detected by Western blot using a plasma membrane protein extraction protocol. The changes in CD83 surface levels in DCs were detected by flow cytometry. Caveolin-1 function was detected in a kidney transplant model. In this study, we demonstrated that caveolin-1 could affect CD83 level during endocytosis in mouse DCs. Caveolin-1 coprecipitates with CD83, as demonstrated by co-IP analysis. TEM morphometric analysis of the entire CD83 distribution associated with internalized caveolin-1 demonstrated a significant interaction in cellular vesicles. sCD83 reduces endogenous CD83 plasma membrane levels, and caveolin-1 knockdown reverts CD83 levels in plasma membrane. sCD83 treatment decreases CD83 surface levels in DCs. siRNA to caveolin-1 in DCs inhibits this effect of sCD83. The effects of sCD83-treated DCs were proved in CD1 mice. Knocking down caveolin-1 in DCs obstructs the effects of sCD83 on kidney transplant. In conclusion, our data indicated that a caveolin-dependent endocytic pathway is involved in CD83 internalization in DCs and that caveolin-1 is involved in the activity of DCs.

语种英语
WOS记录号WOS:000357760500015
资助机构Chinese 5451 project of Henan Province Health Department
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54305
专题北京大学第一临床医学院_泌尿外科
作者单位1.Zhengzhou Univ, Affiliated Hosp 2, Zhengzhou 450052, Peoples R China
2.Univ Western Ontario, Western Sci Ctr, London, ON N6A 4G5, Canada
3.Peking Univ, Hosp 1, Dept Urol, Androl Ctr, Beijing 100871, Peoples R China
4.Univ Western Ontario, Lawson Hlth Res Inst, Crit Illness Res, London, ON N6A 4G5, Canada
推荐引用方式
GB/T 7714
Yang, Yuejing,Xin, Zhongcheng,Chu, Jingjia,et al. Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells[J]. CELL TRANSPLANTATION,2015,24(7):1395-1404.
APA Yang, Yuejing,Xin, Zhongcheng,Chu, Jingjia,Li, Na,&Sun, Tao.(2015).Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells.CELL TRANSPLANTATION,24(7),1395-1404.
MLA Yang, Yuejing,et al."Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells".CELL TRANSPLANTATION 24.7(2015):1395-1404.
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