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学科主题: 药学
题名:
Procaterol but Not Dexamethasone Protects 16HBE Cells From H2O2-Induced Oxidative Stress
作者: Deng, Zheng1,2; Zhou, Jun-Jun1,2; Sun, Shuang-Yong1,2; Zhao, Xin1,2; Sun, Yi1,2; Pu, Xiao-Ping1,2
关键词: oxidative stress ; procaterol ; dexamethasone ; nuclear factor kappa-B ; hydrogen peroxide
刊名: JOURNAL OF PHARMACOLOGICAL SCIENCES
发表日期: 2014-05-01
DOI: 10.1254/jphs.13206FP
卷: 125, 期:1, 页:39-50
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: OBSTRUCTIVE PULMONARY-DISEASE ; BRONCHIAL EPITHELIAL-CELLS ; FACTOR-KAPPA-B ; HYDROGEN-PEROXIDE ; RHINOVIRUS INFECTION ; APOPTOSIS ; ASTHMA ; ACTIVATION ; EXPRESSION ; KINASE
英文摘要:

Oxidative stress is an important pathophysiological factor of asthma and chronic obstructive pulmonary disease (COPD). We hypothesized that procaterol and dexamethasone might treat inflammation through inhibiting oxidative stress in vitro. This study evaluated procaterol and dexamethasone in the hydrogen peroxide (H2O2)-induced immortal human bronchial epithelial cell model of oxidative stress and investigated the underlying mechanisms. Results showed that exposure to 125 mu M H2O2 for 2 h led to a 50% reduction in the cell viability, significantly increased the percentage of apoptosis, and elevated levels of malondialdehyde and reactive oxygen species. Pretreatment with procaterol (25 200 nM) could reduce these effects in a dose-dependent manner. In contrast, pretreatment with dexamethasone (100 nM, 1000 nM) was inefficient. Pretreatment with procaterol plus dexamethasone (100 nM procaterol + 1000 nM dexamethasone) was effective, but the combined effect was not more effective than the sole pretreatment with 100 nM procaterol. The nuclear factor kappa-B (NF-kappa B) pathway was involved in the pathogenic mechanisms of H2O2. Procaterol may indirectly inhibit H2O2-induced activation of the NF-kappa B pathway due to its capability of antioxidation. Glucocorticoids may be not recommended to treat asthma or COPD complicated with severe oxidative stress.

语种: 英语
所属项目编号: 2012ZX09103201-042
项目资助者: National Major Scientific and Technological Special Project for "Significant New Drugs Development" during the Twelfth Five-year Plan Period ; Research Fund of the State Key Laboratory of Natural and Biomimetic Drugs, Peking University, People&prime ; s Republic of China
WOS记录号: WOS:000336556600003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54315
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China

Recommended Citation:
Deng, Zheng,Zhou, Jun-Jun,Sun, Shuang-Yong,et al. Procaterol but Not Dexamethasone Protects 16HBE Cells From H2O2-Induced Oxidative Stress[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2014,125(1):39-50.
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