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Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis
Zhang, Shuyu1; Yin, Ting2; Ling, Xiaomei1; Liang, Hong2; Zhao, Yuying2
关键词Capillary Zone Electrophoresis Interactions Millettia Speciosa Champ. Thrombin
刊名ELECTROPHORESIS
2008-08-01
DOI10.1002/elps.200800174
29期:16页:3391-3397
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TOOL
英文摘要

In Chinese medicine Suberect Spatholobus Stem is used to treat menoxenia, blood deficiency, numb paralyses, and so on. In folk, Millettia speciosa is often used as a substitute for Suberect Spatholobus Stem in some areas but it has not been reported whether M. speciosa is the eligible substitute for Suberect Spatholobus Stem or not till now. In this study, a capillary zone electrophoretic method was applied to determinate the interactions between natural products isolated from M. speciosa Champ. and thrombin for the first time. Both qualitative and quantitative characterizations of the molecule-enzyme binding were determined. Twenty ingredients were isolated from M. speciosa Champ. and the results showed that compared with positive and negative control, the compounds YT-1, YT-2, YT-3, YT-8, YT-9, YT-10, YT-11, YT-12, YT-14, YT-15, YT-16, and YT-20 interacted with thrombin while the other eight had no binding to thrombin. The binding constants of the interaction between compounds and thrombin were calculated by the Scatchard analysis formula. Because M. speciosa contains these compounds which have different levels of anticoagulant activity, it may be the eligible substitute for Suberect Spatholobus Stem.

语种英语
WOS记录号WOS:000258856900014
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54317
专题北京大学药学院_化学生物学系
北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, Shuyu,Yin, Ting,Ling, Xiaomei,et al. Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis[J]. ELECTROPHORESIS,2008,29(16):3391-3397.
APA Zhang, Shuyu,Yin, Ting,Ling, Xiaomei,Liang, Hong,&Zhao, Yuying.(2008).Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis.ELECTROPHORESIS,29(16),3391-3397.
MLA Zhang, Shuyu,et al."Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis".ELECTROPHORESIS 29.16(2008):3391-3397.
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