IR@PKUHSC  > 北京大学基础医学院  > 病理学系
学科主题基础医学
P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells
Li, W-H1,2; Qiu, Y.1,2; Zhang, H-Q1,3; Liu, Y.1,2; You, J-F1,2; Tian, X-X1,2; Fang, W-G1,2
关键词P2Y2 receptor invasion metastasis prostate cancer extracellular ATP EMT
刊名BRITISH JOURNAL OF CANCER
2013-09-17
DOI10.1038/bjc.2013.484
109期:6页:1666-1675
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of China
研究领域[WOS]Oncology
关键词[WOS]PURINERGIC RECEPTORS ; EXTRACELLULAR ATP ; CARCINOMA-CELLS ; TUMOR-CELLS ; GROWTH ; PROTEIN ; INTERLEUKIN-8 ; EXPRESSION ; MIGRATION ; IDENTIFICATION
英文摘要

Background: Our previous study demonstrated that extracellular adenosine 5′-triphosphate (ATP) stimulated prostate cancer cell invasion via P2Y receptors. However, the purinergic receptor subtype(s) involved in this process remains unclear. Here we aimed to determine whether P2Y2, one subtype of P2Y receptors, was involved in the invasion and metastasis of prostate cancer cells, and elucidated the underlying mechanism.

Methods: RNAi was introduced to silence the expression of P2Y2. In vitro invasion and migration assays and in vivo experiments were carried out to examine the role of P2Y2 receptor in cell invasion and metastasis. cDNA microarray was performed to identify the differentially expressed genes downstream of ATP treatment.

Results: P2Y2 was significantly expressed in the prostate cancer cells. Knockdown of P2Y2 receptor suppressed cell invasion and metastasis in vitro and in vivo. Further experiments identified that ATP could promote IL-8 and Snail expression and inhibit E-cadherin and Claudin-1 expression. Knockdown of P2Y2 receptor affected the expression of these EMT/invasion-related genes in vitro and in vivo.

Conclusion: P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells via some EMT/invasion-related genes. Thereby, P2Y2 receptor could be a potential therapeutic target for the treatment of prostate cancer.

语种英语
所属项目编号30971152 ; 2010CB529402
资助者National Natural Science Foundation of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Ministry of Science and Technology of China
WOS记录号WOS:000324812600037
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54335
Collection北京大学基础医学院_病理学系
作者单位1.Peking Univ Hlth Sci Ctr, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100191, Peoples R China
2.Peking Univ Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
3.Peking Univ Hlth Sci Ctr, Dept Anat Histol & Embryol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, W-H,Qiu, Y.,Zhang, H-Q,et al. P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells[J]. BRITISH JOURNAL OF CANCER,2013,109(6):1666-1675.
APA Li, W-H.,Qiu, Y..,Zhang, H-Q.,Liu, Y..,You, J-F.,...&Fang, W-G.(2013).P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells.BRITISH JOURNAL OF CANCER,109(6),1666-1675.
MLA Li, W-H,et al."P2Y2 receptor promotes cell invasion and metastasis in prostate cancer cells".BRITISH JOURNAL OF CANCER 109.6(2013):1666-1675.
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