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学科主题: 临床医学
题名:
Urinary proteomics analysis for sepsis biomarkers with iTRAQ labeling and two-dimensional liquid chromatography-tandem mass spectrometry
作者: Su, Longxiang1,2,3,6; Zhou, Ruo4; Liu, Changting3; Wen, Bo4; Xiao, Kun1,6; Kong, Weijing5; Tan, Fengji4; Huang, Yingyu4; Cao, Lichao4; Xie, Lixin1,6
关键词: Sepsis ; urine ; proteomics ; biomarkers
刊名: JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
发表日期: 2013-03-01
DOI: 10.1097/TA.0b013e31828272c5
卷: 74, 期:3, 页:940-945
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Critical Care Medicine ; Surgery
研究领域[WOS]: General & Internal Medicine ; Surgery
关键词[WOS]: INFLAMMATORY RESPONSE SYNDROME ; SEPTIC SHOCK ; DYSFUNCTION SYNDROME ; INJURY ; COMPLEMENT ; CADHERIN ; FAILURE ; CERULOPLASMIN ; ENDOTOXIN ; PROTEIN
英文摘要:

BACKGROUND: Proteomics has only recently been applied to the field of critical care research. Sepsis is a major factor contributing to intensive care unit admissions and deaths. The purpose of this study was to screen potential urinary biomarkers for sepsis using A proteomics approach.

METHODS: Fifteen sepsis and 15 systemic inflammatory response syndrome patients were involved in this study. Urinary proteins were identified by isobaric tag for relative and absolute quantitation coupled with liquid chromatography-tandem mass spectrometry. Mass spectroscopy analysis was performed with the Mascot software and the International Protein Index. Bioinformatics analyses were performed using the hierarchy cluster analysis, the STRING software, the Gene Ontology, and the Kyoto Encyclopedia of Genes and Genome database.

RESULTS: One hundred thirty proteins were identified, and 34 differentially expressed proteins were selected (fold change, >1.5). On the basis of the Gene Ontology and the Kyoto Encyclopedia of Genes and Genome database, these 34 proteins were identified to be involved in inflammation, immunity, and structural or cytoskeletal processes. Five proteins were selected by a protein-protein interaction network for sepsis differentiation: cadherin 1, haptoglobin, complement 3, alpha-1-antitrypsin, and ceruloplasmin.

CONCLUSION: Urinary proteomics may represent a suitable approach for sepsis-related research. The detection of urinary biomarkers is expected to become a noninvasive and acceptable method, which facilitates the close surveillance of diseases and reduces medical costs. (J Trauma Acute Care Surg. 2013;74: 940-945. Copyright (c) 2013 by Lippincott Williams & Wilkins)

语种: 英语
WOS记录号: WOS:000316321900045
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54337
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Nankai Univ, Coll Med, Tianjin 300071, Peoples R China
2.Beijing Genom Inst, Shenzhen, Peoples R China
3.Chinese Peoples Liberat Army Gen Hosp, Dept Resp Med, Hainan Branch, Sanya 572013, Hainan Province, Peoples R China
4.Chinese Peoples Liberat Army Gen Hosp, Nanlou Resp Dis Dept, Beijing, Peoples R China
5.Peking Univ, Dept Pediat, Hosp 1, Beijing 100871, Peoples R China
6.Chinese Peoples Liberat Army Gen Hosp, Dept Resp Med, Beijing, Peoples R China

Recommended Citation:
Su, Longxiang,Zhou, Ruo,Liu, Changting,et al. Urinary proteomics analysis for sepsis biomarkers with iTRAQ labeling and two-dimensional liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF TRAUMA AND ACUTE CARE SURGERY,2013,74(3):940-945.
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