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学科主题: 基础医学
题名:
Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein
作者: Wu, CX; Zhao, WP; Kishi, H; Dokan, J; Jin, ZX; Wei, XC; Yokoyama, KK; Muraguchi, A
关键词: RAG-2 ; promoter ; MAZ ; Pax-5 ; c-Myb ; LEF-1
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-05-14
DOI: 10.1016/j.bbrc.2004.03.159
卷: 317, 期:4, 页:1096-1102
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: IMMATURE B-CELLS ; V(D)J RECOMBINATION ; GENE-EXPRESSION ; T-CELL ; C-MYB ; TRANSCRIPTIONAL REGULATION ; CHROMATIN STRUCTURE ; CIS-ELEMENTS ; MAZ ; BINDING
英文摘要:

Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during the lymphocyte development. Our previous study showed that the -41 to -17 nucleotides (nt) 5′-up-stream region of mouse RAG-2 were pre-requisite for the core promoter activity and that Pax-5/c-Myb/LEF-1 protein-protein complex was responsible for its activity in immature B cells. In this study, we show that the -65/-42 sequence, the non-conserved sequence between human and mouse RAG-2 promoter, is necessary for the full promoter activity for mouse RAG-2. Electrophoresis mobility shift assay revealed that Myc-associated zinc finger protein (MAZ) as well as SPI/3 binds a GA box in this region. Using chromatin immunoprecipitation, we show that MAZ binds the RAG-2 promoter region in pre-B cells. Furthermore, we show that MAZ synergistically activates the murine RAG-2 promoter with Pax-5/c-Myb/LEF-1 complex. These results first demonstrate that MAZ participates in activation of mouse RAG-2 promoter. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000221174900019
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54362
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Toyama Med & Pharmaceut Univ, Fac Med, Dept Immunol, Toyama 9300194, Japan
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
3.RIKEN, Inst Phys & Chem Res, Tsukuba Inst, BioResource Ctr,Gene Engn Div, Tsukuba, Ibaraki 3050074, Japan

Recommended Citation:
Wu, CX,Zhao, WP,Kishi, H,et al. Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,317(4):1096-1102.
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