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Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell death
Bao, JF; Wu, RG; Zhang, XP; Song, Y; Li, CL
关键词Pc12 Cells Melatonin 1-methyl-4-phenylpyridinium Glucose
刊名ACTA PHARMACOLOGICA SINICA
2005
DOI10.1117/j.1745-7254.2005.00004.x
26期:1页:117-123
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE MPTP ; MITOCHONDRIAL RESPIRATION ; PARKINSONS-DISEASE ; MOUSE-BRAIN ; APOPTOSIS ; 1-METHYL-4-PHENYL-1,2,5,6-TETRAHYDROPYRIDINE ; NEUROTOXICITY ; INHIBITION ; TOXICITY ; GROWTH
英文摘要

Aim: To explore the effect of melatonin on PC 12 cell death induced by 1-methyl-4-phenylpyridinium (MPP(+)). Methods: MTT assay, lactate dehydrogenase (LDH) efflux assay, and immunohistochemistry methods were used to measure neurotoxicity of PC12 cells treated acutely with MPP(+) in low glucose and high glucose conditions, and to assess the neuroprotective effect of melatonin on PC12 cell death induced by MPP(+). Results: In a low glucose condition, MPP(+) significantly induced PC12 cell death, which showed time and concentration dependence. In a serum-free low glucose condition, the percentages of viability of cells treated with MPP(+) for 12, 24, 48, 72, and 96 h were 85.1%, 75.4%, 64.9%, 28.15%, and 9%, respectively. The level of LDH in the culture medium increased and tyrosine hydroxylase positive (TH(+)) cell count decreased. However, in a serum-free high glucose condition, MPP(+) did not significantly induce PC 12 cell death compared with control at various concentrations and time regimens. When the cells were preincubated with melatonin 250 mumol/L for 48, 72, and 96 h in a serum-free low glucose condition, cell survival rate significantly increased to 78.1%, 58.8%, and 31.6%, respectively. Melatonin abolished the LDH leakage of cells treated with MPP(+) and increased TH(+) cells count. Conclusion: MPP(+) caused concentration-dependent PC12 cell death. The level of glucose was an important factor to MPP(+) induced dopaminergic PC12 cell death. Low glucose level could potentiate MPP(+) toxicity, while high glucose level could reduce the toxicity. In addition, melatonin attenuated PC12 cell death induced by MPP(+).

语种英语
WOS记录号WOS:000226426600018
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54365
专题北京大学药学院_分子与细胞药理学系
作者单位1.Beijing Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Beijing Univ, Hlth Sci Ctr, Dept Med Psychol, Beijing 100083, Peoples R China
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GB/T 7714
Bao, JF,Wu, RG,Zhang, XP,et al. Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell death[J]. ACTA PHARMACOLOGICA SINICA,2005,26(1):117-123.
APA Bao, JF,Wu, RG,Zhang, XP,Song, Y,&Li, CL.(2005).Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell death.ACTA PHARMACOLOGICA SINICA,26(1),117-123.
MLA Bao, JF,et al."Melatonin attenuates 1-methyl-4-phenylpyridinium-induced PC12 cell death".ACTA PHARMACOLOGICA SINICA 26.1(2005):117-123.
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