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学科主题: 基础医学
题名:
Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients
作者: Dong, XY; Su, YR; Qian, XP; Yang, XA; Pang, XW; Wu, HY; Chen, WF
关键词: CT antigen ; hepatocellular carcinoma ; antibody response
刊名: BRITISH JOURNAL OF CANCER
发表日期: 2003-07-21
DOI: 10.1038/sj.bjc.6601062
卷: 89, 期:2, 页:291-297
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CANCER/TESTIS ANTIGENS ; TUMOR-SUPPRESSOR ; IMMUNE-RESPONSES ; MAGE FAMILY ; GENE ; TESTIS ; EXPRESSION ; CANCER ; PROTEIN ; LOCALIZATION
英文摘要:

FATE and TPTE genes were originally reported to be specifically expressed in the adult testis. We searched for the databases of Unigene and serial analysis of gene expression ( SAGE) implying that these two gene transcripts might also be expressed in tumours. Herein, we demonstrated that FATE and TPTE mRNA transcripts were expressed in different histological types of tumours and normal testis. Both are cancer-testis (CT) antigens and renamed as FATE/BJ-HCC-2 and TPTE/BJ-HCC-5, respectively. Comparison at nucleotide sequence, the FATE/BJ-HCC-2 cDNA, was identical to that of FATE, whereas the TPTE/BJ-HCC-5 was found to have two isoforms in both cancers and testis: one was identical in cDNA sequence to TPTE, encoding a protein of 551 amino acids, and the other variant lacked an exon of 54 bp, encoding a protein of 533 amino acids. The mRNA expression was analysed by RT-PCR and real-time PCR. FATE/BJ-HCC-2 mRNA was detected in 66% ( 41 out of 62) in hepatocellular carcinoma (HCC) samples and 21% ( three out of 14) in colon cancer samples, whereas the TPTE/BJ-HCC-5 mRNA was detected in 39% ( 24 out of 62) and 36% ( five out of 14) in HCC and non-small lung cancer samples, respectively. The recombinant proteins were prepared and the reactivity of allogenic sera to these two antigens was screened. The frequency of antibody response against FATE/BJ-HCC-2 and TPTE/BJ-HCC-5 proteins was 7.3% ( three out of 41) and 25.0% ( six out of 24), respectively, in HCC patients bearing respective gene transcripts. Therefore, FATE/BJ-HCC-2 and TPTE/BJ-HCC-5 are the novel CT antigens capable of eliciting antibody response in cancer patients.

语种: 英语
WOS记录号: WOS:000184318000016
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54377
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Dong, XY,Su, YR,Qian, XP,et al. Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients[J]. BRITISH JOURNAL OF CANCER,2003,89(2):291-297.
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