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学科主题基础医学
Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients
Dong, XY; Su, YR; Qian, XP; Yang, XA; Pang, XW; Wu, HY; Chen, WF
关键词CT antigen hepatocellular carcinoma antibody response
刊名BRITISH JOURNAL OF CANCER
2003-07-21
DOI10.1038/sj.bjc.6601062
89期:2页:291-297
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CANCER/TESTIS ANTIGENS ; TUMOR-SUPPRESSOR ; IMMUNE-RESPONSES ; MAGE FAMILY ; GENE ; TESTIS ; EXPRESSION ; CANCER ; PROTEIN ; LOCALIZATION
英文摘要

FATE and TPTE genes were originally reported to be specifically expressed in the adult testis. We searched for the databases of Unigene and serial analysis of gene expression ( SAGE) implying that these two gene transcripts might also be expressed in tumours. Herein, we demonstrated that FATE and TPTE mRNA transcripts were expressed in different histological types of tumours and normal testis. Both are cancer-testis (CT) antigens and renamed as FATE/BJ-HCC-2 and TPTE/BJ-HCC-5, respectively. Comparison at nucleotide sequence, the FATE/BJ-HCC-2 cDNA, was identical to that of FATE, whereas the TPTE/BJ-HCC-5 was found to have two isoforms in both cancers and testis: one was identical in cDNA sequence to TPTE, encoding a protein of 551 amino acids, and the other variant lacked an exon of 54 bp, encoding a protein of 533 amino acids. The mRNA expression was analysed by RT-PCR and real-time PCR. FATE/BJ-HCC-2 mRNA was detected in 66% ( 41 out of 62) in hepatocellular carcinoma (HCC) samples and 21% ( three out of 14) in colon cancer samples, whereas the TPTE/BJ-HCC-5 mRNA was detected in 39% ( 24 out of 62) and 36% ( five out of 14) in HCC and non-small lung cancer samples, respectively. The recombinant proteins were prepared and the reactivity of allogenic sera to these two antigens was screened. The frequency of antibody response against FATE/BJ-HCC-2 and TPTE/BJ-HCC-5 proteins was 7.3% ( three out of 41) and 25.0% ( six out of 24), respectively, in HCC patients bearing respective gene transcripts. Therefore, FATE/BJ-HCC-2 and TPTE/BJ-HCC-5 are the novel CT antigens capable of eliciting antibody response in cancer patients.

语种英语
WOS记录号WOS:000184318000016
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54377
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Dong, XY,Su, YR,Qian, XP,et al. Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients[J]. BRITISH JOURNAL OF CANCER,2003,89(2):291-297.
APA Dong, XY.,Su, YR.,Qian, XP.,Yang, XA.,Pang, XW.,...&Chen, WF.(2003).Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients.BRITISH JOURNAL OF CANCER,89(2),291-297.
MLA Dong, XY,et al."Identification of two novel CT antigens and their capacity to elicit antibody response in hepatocellular carcinoma patients".BRITISH JOURNAL OF CANCER 89.2(2003):291-297.
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