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学科主题: 基础医学
题名:
High performance liquid chromatographic determination of 1.2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE), a novel organoselenium compound, in dog plasma using pre-column derivatization and its application in pharmacokinetic study
作者: Zhou, Hai-Yan; Dou, Gui-Fang; Meng, Zhi-Yun; Lou, Ya-Qing; Zhang, Guo-Liang
关键词: 1,2[bis(1,2-Benzisoselenazolone-3(2H)-ketone)]-ethane(BBSKE) ; 2-mercaptoethanol ; pre-column derivatization ; high performance liquid chromatography (HPLC)
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-06-01
DOI: 10.1016/j.jchromb.2007.02.040
卷: 852, 期:1-2, 页:617-624
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: THIOREDOXIN REDUCTASE INHIBITOR ; GLUTATHIONE-PEROXIDASE ; CELL-LINES ; EBSELEN ; APOPTOSIS ; MECHANISM ; PZ-51
英文摘要:

A novel HPLC-UV method with pre-column derivatization by using 2-mercaptoethanol was established for determination of 1,2-[bis(1,2benzisoselenazolone-3(2H)-ketone)] -ethane (BBSKE) in dog plasma. The derivatives were identified by mass spectrometry. The method had a good linear range of 0.05-2 mu g/ml (r(2) = 0.9995). The lower limit of quantification (LOQ) was 0.05 mu g/ml. The precision and accuracy were less than 7%. After dosing of BBSKE (30 mg/kg, p.o. and 0.79 mg/kg, i.v.) in dogs, AUC(0-t), were 5.72 +/- 2.42 and 1.35 +/- 0.41 mu g h/ml; t(1/2) were 4.6 +/- 2.1 and 1.7 +/- 0.6 h, respectively. The method was successfully applied to the pharmacokinetic study in dogs. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000247286700085
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54414
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Beijing Inst Transfus Med, Beijing 100850, Peoples R China
2.Beijing Univ, Basic Med Sch, Dept Pharmacol, Beijing 100083, Peoples R China

Recommended Citation:
Zhou, Hai-Yan,Dou, Gui-Fang,Meng, Zhi-Yun,et al. High performance liquid chromatographic determination of 1.2-[bis(1,2-benzisoselenazolone-3(2H)-ketone)]-ethane (BBSKE), a novel organoselenium compound, in dog plasma using pre-column derivatization and its application in pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,852(1-2):617-624.
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