北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Expression of P-450(c11 beta) in Adrenal Aldosterone-producing Adenomas and Nodular Hyperplasia Tissues
作者: Fang, Yujiang1,2,3,4; Zhao, Lei5; Chen, Shuang6; Cui, Xijun7; Zang, Meifu4; Chen, Songsen8; Di, Xu8
关键词: Aldosteronism ; adrenocortical adenomas ; nodular hyperplasia P-450(c11 beta)
刊名: CLINICAL LABORATORY
发表日期: 2011
卷: 57, 期:3-4, 页:245-251
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: STEROIDOGENIC ENZYMES ; COMPUTED-TOMOGRAPHY ; HYPERALDOSTERONISM ; CT
英文摘要:

Background: Our previous study suggests that decreased P-450(c11 beta) expression correlated with the overproduction of aldosterone in APA and nodular hyperplasia in patients with primary aldosteronism. This study was performed to further investigate if P-450(c11 beta) contributes to the overproduction of aldosterone in APA and nodular hyperplasia tissues

Methods: Total RNA and protein were extracted from 7 cases of APA tissue, 3 nodular hyperplasia tissues, 7 normal adrenal glands. P-450(c11 beta) mRNA was examined by dot blot and confirmed by Northern blot analysis and by realtime PCR. Protein expression level of P-450(c11 beta) was also investigated by immunohistochemical staining and confirmed by Western blot.

Results: The relative expression level of P-450(c11 beta) mRNA to beta-actin in APA, nodular hyperplasia and the normal adrenal gland group are 47+/-22 %, 55+/-13 %, 64+/-16 % respectively by dot blot and are 94+/-18 %, 101+/-20 %, 112+/-62 % respectively by Northern blot. These results are further confirmed by realtime PCR. This result was also supported by the relative protein expression level of P-450(c11 beta) to beta-actin, which are 118+/-15 %, 107+/-32 %, 108+/-22 % respectively evaluated by Western blot. There was no significant difference in protein expression level of P-450(c11 beta) among the normal adrenal gland tissues, APA and adrenal nodular hyperplasia tissue, either (P > 0.05).

Conclusions: These results suggest that P-450(c11 beta) is not a key contributor to the overproduction of aldosterone in APA and nodular hyperplasia and can not be considered as a potential marker to differentiate between them in patients with primary aldosteronism. (Clin. Lab. 2011;57:245-251)

语种: 英语
WOS记录号: WOS:000290595200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54416
Appears in Collections:北京大学第三临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Missouri, Dept Internal Med, Columbia, MO 65211 USA
2.Harry S Truman Mem Vet Hosp, Dept Vet Affairs, Res Serv, Columbia, MO 65201 USA
3.Peking Univ, Hosp 3, Dept Urol, Beijing 100871, Peoples R China
4.Pekin Union Med Coll Hosp, Dept Urol, Beijing, Peoples R China
5.Wannan Med Coll, Affiliated Hosp 2, Dept Internal Med, Wuhu, Peoples R China
6.Mudanjiang Med Coll, Dept Clin Med, Mudanjiang, Peoples R China
7.Peoples Hosp Ningjin, Dept Surg, Ningjin, Peoples R China
8.Chinese Acad Med Sci, Dept Biochem & Mol Biol, Beijing 100037, Peoples R China

Recommended Citation:
Fang, Yujiang,Zhao, Lei,Chen, Shuang,et al. Expression of P-450(c11 beta) in Adrenal Aldosterone-producing Adenomas and Nodular Hyperplasia Tissues[J]. CLINICAL LABORATORY,2011,57(3-4):245-251.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Fang, Yujiang]'s Articles
[Zhao, Lei]'s Articles
[Chen, Shuang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Fang, Yujiang]‘s Articles
[Zhao, Lei]‘s Articles
[Chen, Shuang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace