北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学公共卫生学院  > 毒理学系  > 期刊论文
学科主题: 公共卫生
题名:
Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods
作者: Zou, Peng; Xing, Lina; Tang, Qiuqiong; Liu, Ran; Hao, Weidong
关键词: Genistein ; Genistin ; Micromass culture ; Teratogenicity ; Whole embryo culture
刊名: FOOD AND CHEMICAL TOXICOLOGY
发表日期: 2012-08-01
DOI: 10.1016/j.fct.2012.05.009
卷: 50, 期:8, 页:2831-2836
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Food Science & Technology ; Toxicology
研究领域[WOS]: Food Science & Technology ; Toxicology
关键词[WOS]: SPRAGUE-DAWLEY RATS ; ISOFLAVONE GENISTEIN ; DIETARY ; CANCER ; BIOAVAILABILITY ; PHYTOESTROGENS ; METABOLITES ; TOXICITY ; WOMEN
英文摘要:

Genistein (GEN) is one kind of phytoestrogen. Several studies have demonstrated the teratogenic potential of GEN in vitro by postimplantation rat whole embryo culture (WEC) assay, but GEN showed no teratogenic effects in vivo even at a dose up to 1000 mg/kg bw/day. The mechanism of such discrepancy is still unclear. Because more than 80% of total genistein (free plus glycoside form) in circulation is its glycoside metabolite, genistin (GIN), we thus hypothesize that genistin is non-teratogenic. To prove this hypothesis, rat whole embryo culture (WEC) and limbud micromass culture methods were applied to compare the teratogenic effects of GEN and GIN on developing embryos in vitro. In WEC assay, we found that the development of embryos was affected by GEN treatment dose-dependently, while GIN-treated embryos displayed slight developmental defects only at the highest dose (222 mu M). In micromass culture assay, the IC50 of cell proliferation and differentiation for GEN were 15.6 and 37.2 mu M, respectively, while neither was influenced by GIN treatment up to 111 mu M. Collectively, our study indicated that GEN showed no teratogenic effects in vivo probably due to its transformation to the non-teratogenic metabolite, GIN. (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30771821 ; 2009ZX09301-010
项目资助者: National Natural Science Foundation of People&prime ; s Republic of China ; National Key Technology Research and Development Program
WOS记录号: WOS:000308255800035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54437
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Dept Toxicol, Sch Publ Hlth, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China

Recommended Citation:
Zou, Peng,Xing, Lina,Tang, Qiuqiong,et al. Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods[J]. FOOD AND CHEMICAL TOXICOLOGY,2012,50(8):2831-2836.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zou, Peng]'s Articles
[Xing, Lina]'s Articles
[Tang, Qiuqiong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zou, Peng]‘s Articles
[Xing, Lina]‘s Articles
[Tang, Qiuqiong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace