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Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods
Zou, Peng; Xing, Lina; Tang, Qiuqiong; Liu, Ran; Hao, Weidong
关键词Genistein Genistin Micromass Culture Teratogenicity Whole Embryo Culture
刊名FOOD AND CHEMICAL TOXICOLOGY
2012-08-01
DOI10.1016/j.fct.2012.05.009
50期:8页:2831-2836
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Food Science & Technology ; Toxicology
资助者National Natural Science Foundation of People&prime ; s Republic of China ; National Key Technology Research and Development Program ; National Natural Science Foundation of People&prime ; s Republic of China ; National Key Technology Research and Development Program
研究领域[WOS]Food Science & Technology ; Toxicology
关键词[WOS]SPRAGUE-DAWLEY RATS ; ISOFLAVONE GENISTEIN ; DIETARY ; CANCER ; BIOAVAILABILITY ; PHYTOESTROGENS ; METABOLITES ; TOXICITY ; WOMEN
英文摘要

Genistein (GEN) is one kind of phytoestrogen. Several studies have demonstrated the teratogenic potential of GEN in vitro by postimplantation rat whole embryo culture (WEC) assay, but GEN showed no teratogenic effects in vivo even at a dose up to 1000 mg/kg bw/day. The mechanism of such discrepancy is still unclear. Because more than 80% of total genistein (free plus glycoside form) in circulation is its glycoside metabolite, genistin (GIN), we thus hypothesize that genistin is non-teratogenic. To prove this hypothesis, rat whole embryo culture (WEC) and limbud micromass culture methods were applied to compare the teratogenic effects of GEN and GIN on developing embryos in vitro. In WEC assay, we found that the development of embryos was affected by GEN treatment dose-dependently, while GIN-treated embryos displayed slight developmental defects only at the highest dose (222 mu M). In micromass culture assay, the IC50 of cell proliferation and differentiation for GEN were 15.6 and 37.2 mu M, respectively, while neither was influenced by GIN treatment up to 111 mu M. Collectively, our study indicated that GEN showed no teratogenic effects in vivo probably due to its transformation to the non-teratogenic metabolite, GIN. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号30771821 ; 2009ZX09301-010
资助者National Natural Science Foundation of People&prime ; s Republic of China ; National Key Technology Research and Development Program ; National Natural Science Foundation of People&prime ; s Republic of China ; National Key Technology Research and Development Program
WOS记录号WOS:000308255800035
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54437
专题北京大学公共卫生学院_毒理学系
作者单位Peking Univ, Dept Toxicol, Sch Publ Hlth, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zou, Peng,Xing, Lina,Tang, Qiuqiong,et al. Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods[J]. FOOD AND CHEMICAL TOXICOLOGY,2012,50(8):2831-2836.
APA Zou, Peng,Xing, Lina,Tang, Qiuqiong,Liu, Ran,&Hao, Weidong.(2012).Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods.FOOD AND CHEMICAL TOXICOLOGY,50(8),2831-2836.
MLA Zou, Peng,et al."Comparative evaluation of the teratogenicity of genistein and genistin using rat whole embryo culture and limbud micromass culture methods".FOOD AND CHEMICAL TOXICOLOGY 50.8(2012):2831-2836.
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