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学科主题基础医学
CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes
Wang, Shanshan2; Wei, Qiong1; Zhu, Tao1; Huang, Jianyong1; Yu, Min2,3; Sha, Yinlin2,3; Xiong, Chunyang1,2; Fang, Jing1,2
关键词CD4+T cell cell counting hydrodynamic focusing fluidic electrodes microfluidics
刊名INTERNATIONAL JOURNAL OF NONLINEAR SCIENCES AND NUMERICAL SIMULATION
2012-09-01
DOI10.1515/ijnsns-2012-0400
13期:5页:311-317
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Multidisciplinary ; Mathematics, Applied ; Mechanics ; Physics, Mathematical
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; National Basic Research Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
研究领域[WOS]Engineering ; Mathematics ; Mechanics ; Physics
关键词[WOS]MICRO-COULTER COUNTER ; MICROFLUIDIC DEVICE ; BACTERIAL-CELLS ; AC-IMPEDANCE ; SPECTROSCOPY ; BIOSENSOR
英文摘要

A practical label-free method for counting CD4+ T cells is proposed on the basis of a microfluidic chip with fluidic electrodes. With the help of hydrodynamic focusing, two sheath flows of KCl solution, serving as electric conductors to replace solid metal electrodes, are used to squeeze the cell suspension. By measuring the electrical impedances between the fluidic electrodes, a linear relationship is found between the logarithmic value of cell concentration and the impedance value (R-2 = 0.97). The detection limit of CD4+ T lymphocytes reaches as low as similar to 10 cells.mu L-1, Both the CD4+ T cell concentration of the nude mice group and that of the normal group were counted to further test the function of the chip system, which indicated that there existed significant differences between these groups, showing the approach is valid for identifying immunodeficiency.

语种英语
所属项目编号2011CB809106 ; 61076112 ; 11072004
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; National Basic Research Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
WOS记录号WOS:000310334900001
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54447
专题基础医学院_生物物理学系
作者单位1.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Mol & Nanobiol Lab, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Wang, Shanshan,Wei, Qiong,Zhu, Tao,et al. CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes[J]. INTERNATIONAL JOURNAL OF NONLINEAR SCIENCES AND NUMERICAL SIMULATION,2012,13(5):311-317.
APA Wang, Shanshan.,Wei, Qiong.,Zhu, Tao.,Huang, Jianyong.,Yu, Min.,...&Fang, Jing.(2012).CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes.INTERNATIONAL JOURNAL OF NONLINEAR SCIENCES AND NUMERICAL SIMULATION,13(5),311-317.
MLA Wang, Shanshan,et al."CD4+ T Cell Counting by Impedance Measurement on a Chip with Fluidic Electrodes".INTERNATIONAL JOURNAL OF NONLINEAR SCIENCES AND NUMERICAL SIMULATION 13.5(2012):311-317.
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