北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 普通外科  > 期刊论文
学科主题: 临床医学
题名:
Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets
作者: Bao, Yang; Xiu, Dian-Rong; Zhang, Li
关键词: Rejection ; surface-enhanced laser desorption ; ionization time-of-flight mass spectrometry (SELDI-TOF MS) ; transplant ; complement component C3f fragment ; complement component 4A (C4A ; anaphylatoxin)
刊名: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
发表日期: 2013-06-01
DOI: 10.1177/0300060513476997
卷: 41, 期:3, 页:628-635
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: RENAL-TRANSPLANTATION ; COMPLEMENT ; PLASMA ; METHOTREXATE ; ASSOCIATION ; C4A
英文摘要:

Objective

Methotrexate and rapamycin demonstrate an additive effect in prolonging cardiac allograft survival in a major histocompatibility complex mismatched rat model. The present study aimed to identify functional proteins involved in the allograft-protective effects of these two agents and reveal potential diagnostic markers for treating rejection.

Methods

Serum samples from heterotopic heart-transplanted LEW(RT-1(1)) rats (either without immunosuppressive treatment or treated with methotrexate alone, rapamycin alone, or methotrexate and rapamycin combined) were analysed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Protein profiles obtained using a weak cation exchange ProteinChip (R) CM-10 array were then analysed using ProteinChip (R) Software.

Results

Of 28 rejection-related proteins identified, isoelectric point and mass information from two potential candidate proteins matched information from the UniProtKB/Swiss-prot database, suggesting them to be complement component C3f fragment and complement component 4A (C4A, anaphylatoxin).

Conclusions

Proteomic analysis revealed 28 proteins as potential diagnostic markers of tissue rejection. Of these, 11 proteins may represent targets relating to the additive effects of methotrexate and rapamycin. Two protein peaks, with mass-to-charge ratios of 1950 Da and 8577 Da, may have potential for use in post-transplant diagnosis of rejection.

语种: 英语
所属项目编号: ZR09-6-02
项目资助者: National Nature Science Fund of China
WOS记录号: WOS:000319943300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54448
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Dept Surg, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Bao, Yang,Xiu, Dian-Rong,Zhang, Li. Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2013,41(3):628-635.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Bao, Yang]'s Articles
[Xiu, Dian-Rong]'s Articles
[Zhang, Li]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Bao, Yang]‘s Articles
[Xiu, Dian-Rong]‘s Articles
[Zhang, Li]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace