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Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets
Bao, Yang; Xiu, Dian-Rong; Zhang, Li
关键词Rejection Surface-enhanced Laser Desorption Ionization Time-of-flight Mass Spectrometry (Seldi-tof Ms) Transplant Complement Component C3f Fragment Complement Component 4a (C4a Anaphylatoxin)
刊名JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
2013-06-01
DOI10.1177/0300060513476997
41期:3页:628-635
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]RENAL-TRANSPLANTATION ; COMPLEMENT ; PLASMA ; METHOTREXATE ; ASSOCIATION ; C4A
英文摘要

Objective

Methotrexate and rapamycin demonstrate an additive effect in prolonging cardiac allograft survival in a major histocompatibility complex mismatched rat model. The present study aimed to identify functional proteins involved in the allograft-protective effects of these two agents and reveal potential diagnostic markers for treating rejection.

Methods

Serum samples from heterotopic heart-transplanted LEW(RT-1(1)) rats (either without immunosuppressive treatment or treated with methotrexate alone, rapamycin alone, or methotrexate and rapamycin combined) were analysed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Protein profiles obtained using a weak cation exchange ProteinChip (R) CM-10 array were then analysed using ProteinChip (R) Software.

Results

Of 28 rejection-related proteins identified, isoelectric point and mass information from two potential candidate proteins matched information from the UniProtKB/Swiss-prot database, suggesting them to be complement component C3f fragment and complement component 4A (C4A, anaphylatoxin).

Conclusions

Proteomic analysis revealed 28 proteins as potential diagnostic markers of tissue rejection. Of these, 11 proteins may represent targets relating to the additive effects of methotrexate and rapamycin. Two protein peaks, with mass-to-charge ratios of 1950 Da and 8577 Da, may have potential for use in post-transplant diagnosis of rejection.

语种英语
WOS记录号WOS:000319943300011
项目编号ZR09-6-02
资助机构National Nature Science Fund of China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54448
专题北京大学第三临床医学院_普通外科
作者单位Peking Univ, Dept Surg, Hosp 3, Beijing 100191, Peoples R China
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Bao, Yang,Xiu, Dian-Rong,Zhang, Li. Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2013,41(3):628-635.
APA Bao, Yang,Xiu, Dian-Rong,&Zhang, Li.(2013).Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets.JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,41(3),628-635.
MLA Bao, Yang,et al."Proteomic profiling of heterotopic heart-transplanted rats using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry: Potential biomarkers and drug targets".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 41.3(2013):628-635.
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