IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室
学科主题临床医学
Novel recurrently mutated genes and a prognostic mutation signature in colorectal cancer
Yu, Jun1,2; Wu, William K. K.1,2; Li, Xiangchun1,2,3; He, Jun1,2,3; Li, Xiao-Xing1,2; Ng, Simon S. M.4; Yu, Chang1,2,3; Gao, Zhibo1,2,3; Yang, Jie3; Li, Miao3; Wang, Qiaoxiu3; Liang, Qiaoyi1,2; Pan, Yi5; Tong, Joanna H.5; To, Ka F.5; Wong, Nathalie5; Zhang, Ning1,2,6; Chen, Jie6; Lu, Youyong7; Lai, Paul B. S.4; Chan, Francis K. L.1,2; Li, Yingrui3; Kung, Hsiang-Fu8; Yang, Huanming3; Wang, Jun3; Sung, Joseph J. Y.1,2
刊名GUT
2015-04-01
DOI10.1136/gutjnl-2013-306620
64期:4页:636-645
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]COMPREHENSIVE MOLECULAR CHARACTERIZATION ; COLON-CANCER ; MICROSATELLITE INSTABILITY ; SOMATIC MUTATION ; SEQUENCING DATA ; BETA-CATENIN ; ACTIVATION ; EXOME ; IDENTIFICATION ; SURVIVAL
英文摘要

Background Characterisation of colorectal cancer (CRC) genomes by next-generation sequencing has led to the discovery of novel recurrently mutated genes. Nevertheless, genomic data has not yet been used for CRC prognostication.

Objective To identify recurrent somatic mutations with prognostic significance in patients with CRC.

Method Exome sequencing was performed to identify somatic mutations in tumour tissues of 22 patients with CRC, followed by validation of 187 recurrent and pathway-related genes using targeted capture sequencing in additional 160 cases.

Results Seven significantly mutated genes, including four reported (APC, TP53, KRAS and SMAD4) and three novel recurrently mutated genes (CDH10, FAT4 and DOCK2), exhibited high mutation prevalence (6-14% for novel cancer genes) and higher-than-expected number of non-silent mutations in our CRC cohort. For prognostication, a five-gene-signature (CDH10, COL6A3, SMAD4, TMEM132D, VCAN) was devised, in which mutation(s) in one or more of these genes was significantly associated with better overall survival independent of tumor-node-metastasis (TNM) staging. The median survival time was 80.4 months in the mutant group versus 42.4 months in the wild type group (p= 0.0051). The prognostic significance of this signature was successfully verified using the data set from the Cancer Genome Atlas study.

Conclusions The application of next-generation sequencing has led to the identification of three novel significantly mutated genes in CRC and a mutation signature that predicts survival outcomes for stratifying patients with CRC independent of TNM staging.

语种英语
WOS记录号WOS:000350800100019
引用统计
被引频次:50[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54468
专题北京大学临床肿瘤学院_分子肿瘤学研究室
作者单位1.Beijing Genom Inst Shenzhen, Shenzhen 518000, Peoples R China
2.Chinese Univ Hong Kong, Dept Med & Therapeut, State Key Lab Digest Dis, Inst Digest Dis, Shatin, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, LKS Inst Hlth Sci, CUHK Shenzhen Res Inst, Shatin, Hong Kong, Peoples R China
4.Chinese Univ Hong Kong, Dept Surg, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Dept Anat & Cellular Pathol, Shatin, Hong Kong, Peoples R China
6.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Gastroenterol, Guangzhou 510275, Guangdong, Peoples R China
7.Peking Univ, Canc Hosp & Inst, Lab Mol Oncol, Beijing 100871, Peoples R China
8.Chinese Univ Hong Kong, Stanley Ho Ctr Emerging Infect Dis, Shatin, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Yu, Jun,Wu, William K. K.,Li, Xiangchun,et al. Novel recurrently mutated genes and a prognostic mutation signature in colorectal cancer[J]. GUT,2015,64(4):636-645.
APA Yu, Jun.,Wu, William K. K..,Li, Xiangchun.,He, Jun.,Li, Xiao-Xing.,...&Sung, Joseph J. Y..(2015).Novel recurrently mutated genes and a prognostic mutation signature in colorectal cancer.GUT,64(4),636-645.
MLA Yu, Jun,et al."Novel recurrently mutated genes and a prognostic mutation signature in colorectal cancer".GUT 64.4(2015):636-645.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yu, Jun]的文章
[Wu, William K. K.]的文章
[Li, Xiangchun]的文章
百度学术
百度学术中相似的文章
[Yu, Jun]的文章
[Wu, William K. K.]的文章
[Li, Xiangchun]的文章
必应学术
必应学术中相似的文章
[Yu, Jun]的文章
[Wu, William K. K.]的文章
[Li, Xiangchun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。