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Identification and characterization of antioxidants from Sophora flavescens
Piao, Xiang-Lan; Piao, Xiang Shu; Kim, Sung Woo; Park, Jeong Hill; Kim, Hyun Young; Cai, Shao-Qing
关键词Sophora Flavescens Antioxidative Activity Hplc/ms Sophoraflavanone g Kurarinone
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2006-09-01
29期:9页:1911-1915
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]OXIDATIVE STRESS ; LIPID-PEROXIDATION ; RADICAL INITIATOR ; VITAMIN-E ; IN-VITRO ; RAT ; OXIDANTS ; DEFENSE ; DISEASE ; OXYGEN
英文摘要

The objectives of this study were to investigate the radical-scavenging activity and protective potential of Sophora flavescens from oxidative damage by the radical generator 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH) in renal epithelial LLC-PK1 cells and to identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH2Cl2, BuOH, and H2O from S. flavescens showed 1,1-diphenyl-2-picrylhyd razyl (DPPH) radical-scavenging effects in a dose-dependent manner (p < 0.01),whereas only the BuOH and CH2Cl2 fractions showed protective effects against LLC-PK, cellular damage induced by AAPH in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. Employing a bioassay-linked HPLC/MS method, the active constituents from the BuOH fraction of S. flavescens were isolated and characterized as sophorallavanone G and kurarinone with potent antioxidant effects against the DPPH radical, with IC50 values of 5.26 and 7.73 mu g/ml, respectively. Moreover, the compounds dose dependently recovered cell viability decreased by AAPH treatment (p < 0.01), suggesting their protective roles against cellular oxidative damage. The results of this study suggest that S. flavescens has excellent antioxidative and kidney-protective potential and that flavonoids from S.flaveseens, i.e., sophoraflavanone G and kurarinone, are the active constituents.

语种英语
WOS记录号WOS:000240970900023
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54499
专题北京大学药学院
作者单位1.Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China
4.Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA
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GB/T 7714
Piao, Xiang-Lan,Piao, Xiang Shu,Kim, Sung Woo,et al. Identification and characterization of antioxidants from Sophora flavescens[J]. BIOLOGICAL &amp; PHARMACEUTICAL BULLETIN,2006,29(9):1911-1915.
APA Piao, Xiang-Lan,Piao, Xiang Shu,Kim, Sung Woo,Park, Jeong Hill,Kim, Hyun Young,&Cai, Shao-Qing.(2006).Identification and characterization of antioxidants from Sophora flavescens.BIOLOGICAL & PHARMACEUTICAL BULLETIN,29(9),1911-1915.
MLA Piao, Xiang-Lan,et al."Identification and characterization of antioxidants from Sophora flavescens".BIOLOGICAL & PHARMACEUTICAL BULLETIN 29.9(2006):1911-1915.
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