北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北大医学部天士力微循环研究中心  > 期刊论文
学科主题: 基础医学
题名:
Early inhibition of HIF-1 alpha with small interfering RNA reduces ischemic-reperfused brain injury in rats
作者: Chen, Chunhua1,4; Hu, Qin1; Yan, Junhao1; Yang, Xiaomei1; Shi, Xianzhong1; Lei, Jiliang1; Chen, Lin2; Huang, Hongyun2; Han, Jingyan3; Zhang, John H.4; Zhou, Changman1,2,3
关键词: Apoptosis ; HIF-1 alpha ; MCAO ; siRNA
刊名: NEUROBIOLOGY OF DISEASE
发表日期: 2009-03-01
DOI: 10.1016/j.nbd.2008.12.010
卷: 33, 期:3, 页:509-517
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: HYPOXIA-INDUCIBLE FACTOR ; CEREBRAL-ARTERY OCCLUSION ; FACTOR-I HIF-1 ; HYPERBARIC-OXYGEN ; APOPTOTIC GENES ; FOCAL ISCHEMIA ; MODEL ; EXPRESSION ; FACTOR-1-ALPHA ; INDUCTION
英文摘要:

Hypoxia-inducible factor-1 (HIF-1) plays an essential role in cerebral ischemia as a proapoptotic factor. We hypothesized that HIF-1 alpha siRNA can protect the brain from ischemic damage by inhibiting HIF-1 alpha induced apoptotic pathway at the RNA level in a rat focal ischemic model. Results showed that treatment with HIF-1 alpha siRNA reduced the infarct volume, decreased mortality, improved neurological deficits and reduced Evans blue extravasation. The expression of HIF-1 alpha mRNA (Real-Time PCR) and protein were significantly silenced and the immunohistochemistry and Western blot revealed the suppression of HIF-1 alpha, VEGF, p53 and Caspase-3. Double fluorescence labeling showed HIF-1 alpha positive immunoreactive materials were partly colocalized with NeuN, p53 and Caspase-3 in the injured cerebral cortex. This study showed that HIF-1 alpha siRNA may protect the ischemic-reperfused neurons in vivo via inhibition of HIF-1 alpha, its downstream VEGF and other apoptotic-related proteins such as p53 and Caspase-3 and may have potentials for the early treatment of ischemic cerebral stroke. (C) 2008 Published by Elsevier Inc.

语种: 英语
所属项目编号: 30672157 ; 20050001123
项目资助者: National Natural Science Foundation of China ; Graduate Student Foundation of China
WOS记录号: WOS:000263748800021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54505
Appears in Collections:基础医学院_北大医学部天士力微循环研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S0969996108003161-main.pdf(2026KB)期刊论文作者接受稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Anat & Embryol, Beijing 100083, Peoples R China
2.Beijing Hongtianji Neurosci Acad, Dept Neurosurg, Beijing 100144, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100083, Peoples R China
4.Loma Linda Univ, Dept Neurosurg, Loma Linda, CA 92350 USA

Recommended Citation:
Chen, Chunhua,Hu, Qin,Yan, Junhao,et al. Early inhibition of HIF-1 alpha with small interfering RNA reduces ischemic-reperfused brain injury in rats[J]. NEUROBIOLOGY OF DISEASE,2009,33(3):509-517.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Chunhua]'s Articles
[Hu, Qin]'s Articles
[Yan, Junhao]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Chunhua]‘s Articles
[Hu, Qin]‘s Articles
[Yan, Junhao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace