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Suppression of tumor growth and metastasis by a vegfr-1 antagonizing peptide identified from a phage display library
An, P; Lei, HT; Zhang, JZ; Song, SM; He, LW; Jin, GL; Liu, XY; Wu, J; Meng, L; Liu, MS; Shou, CC
关键词Tumor Angiogenesis Peptide Suppression Vegfr-1
刊名INTERNATIONAL JOURNAL OF CANCER
2004-08-20
DOI10.1002/ijc.20214
111期:2页:165-173
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]FACTOR RECEPTOR FLT-1 ; TYROSINE KINASE ; ANGIOGENESIS ; CANCER ; EXPRESSION ; DOMAIN ; CELLS ; INHIBITION ; ANTIBODIES ; THERAPY
英文摘要

Although the VEGF-Flk-1-pathway has been known as the major driving force of angiogenesis, new evidence has shown that VEGFR-1/Flt-1 plays important roles during the neovascularization under pathological conditions including tumor, atherosclerosis and arthritis. In search of Flt-1 receptor antagonizing peptides, we screened a phage display 12-merpeptide library with recombinant Flt-1 protein. Seven candidate peptides were identified that specifically bound to VEGF receptor Flt-1, of which peptide F56 (WHSDMEWWYLLG) almost abolished VEGF binding to receptor Flt-1 in vitro. In vivo, F56 fused with DHFR (DHFR-F56) inhibited angiogenesis in a CAM assay. Moreover, DHFR-F56 significantly inhibited the growth of nodules of human gastric cancer cell line MGC-803 in BALB/c nude mice. Histological analyses showed that necrosis of the implanted tumor was markedly enhanced following treatment with DHFR-F56. In the severe combined immunodeficiency disease (SCID) mouse model for studying metastasis of the human breast cancer cell line BICR-H1, synthetic peptide F56 significantly inhibited tumor growth and lung metastases. Taken together, our results have demonstrated that peptide F56, as a Flt-1 receptor antagonist, fulfilled the antiangiogenic and antimetastatic effects by specifically interfering with the interaction between VEGF and receptor Flt-1. Thus, short peptide F56 may have clinical potential in tumor therapy. (C) 2004 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000222646400001
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被引频次:46[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54508
专题北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
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GB/T 7714
An, P,Lei, HT,Zhang, JZ,et al. Suppression of tumor growth and metastasis by a vegfr-1 antagonizing peptide identified from a phage display library[J]. INTERNATIONAL JOURNAL OF CANCER,2004,111(2):165-173.
APA An, P.,Lei, HT.,Zhang, JZ.,Song, SM.,He, LW.,...&Shou, CC.(2004).Suppression of tumor growth and metastasis by a vegfr-1 antagonizing peptide identified from a phage display library.INTERNATIONAL JOURNAL OF CANCER,111(2),165-173.
MLA An, P,et al."Suppression of tumor growth and metastasis by a vegfr-1 antagonizing peptide identified from a phage display library".INTERNATIONAL JOURNAL OF CANCER 111.2(2004):165-173.
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