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Reverse-Phase High-Performance Liquid-Chromotographic DAD Method for Simultaneous Determination of Five Isoflavones in Millettia nitida var. hirsutissima
Xiang, Cheng2; Liang, Hong1,2; Wang, Bin2; Zhao, Yu-Ying2
关键词Hplc-dad Isoflavones Millettia Nitida Var Hirsutissima Quantitative Determination
刊名ANALYTICAL LETTERS
2009
DOI10.1080/00032710902890413
42期:8页:1148-1156
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLAVONOIDS ; CELLS
英文摘要

A reverse-phase high-performance liquid-chromatographic photodiode array detection (DAD) method has been developed and validated for the simultaneous determination of five isoflavones in Millettia nitida var. hirsutissima: hirsutissimiside B, sphaerobioside, lanceolarin, formononetin, and afromosin. The method utilized a C18 column (4m, 2504.6mm) with an acetonitrile and aqueous H3PO4 gradient and ultraviolet detection at 259nm. The recovery of the method is 94.1-105.9%, and linearity (r0.9996) is obtained for all the isoflavones.

语种英语
WOS记录号WOS:000266437100008
项目编号20432030 ; 985-2-063-112
资助机构National Natural Science Foundation of China ; Program for Changjiang Scholars and Innovation Team in University
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54511
专题北京大学药学院_天然药物学系
北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Hlth Sci Ctr, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Xiang, Cheng,Liang, Hong,Wang, Bin,et al. Reverse-Phase High-Performance Liquid-Chromotographic DAD Method for Simultaneous Determination of Five Isoflavones in Millettia nitida var. hirsutissima[J]. ANALYTICAL LETTERS,2009,42(8):1148-1156.
APA Xiang, Cheng,Liang, Hong,Wang, Bin,&Zhao, Yu-Ying.(2009).Reverse-Phase High-Performance Liquid-Chromotographic DAD Method for Simultaneous Determination of Five Isoflavones in Millettia nitida var. hirsutissima.ANALYTICAL LETTERS,42(8),1148-1156.
MLA Xiang, Cheng,et al."Reverse-Phase High-Performance Liquid-Chromotographic DAD Method for Simultaneous Determination of Five Isoflavones in Millettia nitida var. hirsutissima".ANALYTICAL LETTERS 42.8(2009):1148-1156.
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