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学科主题: 基础医学
题名:
LIF maintains progenitor phenotype of endothelial progenitor cells via Kruppel-like factor 4
作者: Li, Xiaoxia1; Song, Yimeng1; Wang, Dawei1; Fu, Chenglai1; Zhu, Zhenjiu1; Han, Yingying1; Li, Chenghong1; Wang, Nanping2; Zhu, Yi1
刊名: MICROVASCULAR RESEARCH
发表日期: 2012-11-01
DOI: 10.1016/j.mvr.2012.07.005
卷: 84, 期:3, 页:270-277
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Peripheral Vascular Disease
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: LEUKEMIA INHIBITORY FACTOR ; EMBRYONIC STEM-CELLS ; SELF-RENEWAL ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; DIFFERENTIATION ; GROWTH ; MOUSE ; PROLIFERATION ; PLURIPOTENCY
英文摘要:

Background: Endothelial progenitor cells (EPCs) participate in post-natal vasculogenesis. Maintaining the preliminary progenitor phenotype and good proliferation capacity of EPCs is key to their use in treating cardiovascular ischemic diseases. However, transcriptional regulation in EPCs remains largely unknown. We investigated the effect of leukemia inhibitory factor (LIF) combined with vascular endothelial growth factor (VEGF) on EPCs and the potential roles of Kruppel-like transcription factors (KLFs).

Methods and results: Co-treatment with LIF and VEGF (100 ng/ml each) (V+L) could increase EPC colony-forming units and CD34 expression, which reflects the EPC progenitor phenotype and alleviated differentiation of EPCs. The effect was associated with Akt activation and increased expression of KLF4. Upregulation of KLF4 induced by V+L could be inhibited by transfection with dominant-negative Akt adenovirus. Furthermore, overexpression of KLF4 in EPCs enhanced the expression of CD34 and alleviated cell differentiation but did not increase the phosphorylation of Akt.

Conclusions: LIF combined with VEGF can maintain the preliminary, progenitor phenotype of EPCs and alleviate cell differentiation by upregulating KLF4, which may provide new insights into transcriptional regulation in EPCs. (C) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 81070113 ; 30600209 ; 30630032 ; 30821001 ; 2010CB912504
项目资助者: National Natural Science Foundation of China ; Major National Basic Research Grant of China
WOS记录号: WOS:000310862200006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54513
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Key Lab Cardiovasc Sci, Minist Educ,Inst Cardiovasc Res, Beijing 100191, Peoples R China

Recommended Citation:
Li, Xiaoxia,Song, Yimeng,Wang, Dawei,et al. LIF maintains progenitor phenotype of endothelial progenitor cells via Kruppel-like factor 4[J]. MICROVASCULAR RESEARCH,2012,84(3):270-277.
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