|Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells|
|Ke, XY; Jia, LP; Wang, J; Wang, DB|
|关键词||B7 Gene Transfection Tumor Immunity|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||COSTIMULATORY MOLECULES ; ANTITUMOR IMMUNITY ; INDUCTION ; ANERGY ; IMMUNOTHERAPY ; ERADICATION ; TOLERANCE ; MECHANISM ; REJECTION ; PREVENTS|
Objective To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti-tumor T cell immune responses.
Methods Examining human leucocyte antigen (HLA) and 137 expressions on 8 human blood malignancies cell lines by flow cytometry. Transfecting B7-1 gene to B7-1 negative (B7(-)) Raji and B7(-) Jurkat cell lines by liposome, and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transfection using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).
Results High level of HLA I and II molecules were expressed in most human blood malignant cell lines examined, and the co-stimulatory factor B7-2 was also highly expressed. in contrast, another member of B7 family: B7-1 was either not expressed or very limitedly expressed in most of these hematopoietic malignant cell lines. Most importantly, transfection of B7-1 gene to B7(-). Raji and B7(-). Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti-tumor T cell activation, which was demonstrated by up regulation of expression of T cell cytokines IL-2, IL-4 and INF-gamma mRNAs after incubation of these tumor cells with T cells for 24 h.
Conclusions B7 co-stimulation plays an important role in anti-tumor immunity. Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potency for activation of anti-tumor T cells. Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoietic malignancies.
|作者单位||1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100083, Peoples R China|
2.Peking Univ, Peoples Hosp, Inst Blood Dis Res, Beijing 100083, Peoples R China
|Ke, XY,Jia, LP,Wang, J,et al. Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells[J]. CHINESE MEDICAL JOURNAL,2003,116(1):78-84.|
|APA||Ke, XY,Jia, LP,Wang, J,&Wang, DB.(2003).Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells.CHINESE MEDICAL JOURNAL,116(1),78-84.|
|MLA||Ke, XY,et al."Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells".CHINESE MEDICAL JOURNAL 116.1(2003):78-84.|