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学科主题临床医学
Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells
Ke, XY; Jia, LP; Wang, J; Wang, DB
关键词B7 Gene Transfection Tumor Immunity
刊名CHINESE MEDICAL JOURNAL
2003
116期:1页:78-84
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]COSTIMULATORY MOLECULES ; ANTITUMOR IMMUNITY ; INDUCTION ; ANERGY ; IMMUNOTHERAPY ; ERADICATION ; TOLERANCE ; MECHANISM ; REJECTION ; PREVENTS
英文摘要

Objective To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti-tumor T cell immune responses.

Methods Examining human leucocyte antigen (HLA) and 137 expressions on 8 human blood malignancies cell lines by flow cytometry. Transfecting B7-1 gene to B7-1 negative (B7(-)) Raji and B7(-) Jurkat cell lines by liposome, and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transfection using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).

Results High level of HLA I and II molecules were expressed in most human blood malignant cell lines examined, and the co-stimulatory factor B7-2 was also highly expressed. in contrast, another member of B7 family: B7-1 was either not expressed or very limitedly expressed in most of these hematopoietic malignant cell lines. Most importantly, transfection of B7-1 gene to B7(-). Raji and B7(-). Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti-tumor T cell activation, which was demonstrated by up regulation of expression of T cell cytokines IL-2, IL-4 and INF-gamma mRNAs after incubation of these tumor cells with T cells for 24 h.

Conclusions B7 co-stimulation plays an important role in anti-tumor immunity. Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potency for activation of anti-tumor T cells. Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoietic malignancies.

语种英语
WOS记录号WOS:000181239800018
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54522
专题北京大学第三临床医学院_血液内科
作者单位1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Blood Dis Res, Beijing 100083, Peoples R China
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GB/T 7714
Ke, XY,Jia, LP,Wang, J,et al. Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells[J]. CHINESE MEDICAL JOURNAL,2003,116(1):78-84.
APA Ke, XY,Jia, LP,Wang, J,&Wang, DB.(2003).Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells.CHINESE MEDICAL JOURNAL,116(1),78-84.
MLA Ke, XY,et al."Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells".CHINESE MEDICAL JOURNAL 116.1(2003):78-84.
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