北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 血液内科  > 期刊论文
学科主题: 临床医学
题名:
Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells
作者: Ke, XY; Jia, LP; Wang, J; Wang, DB
关键词: B7 ; gene transfection ; tumor immunity
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2003
卷: 116, 期:1, 页:78-84
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: COSTIMULATORY MOLECULES ; ANTITUMOR IMMUNITY ; INDUCTION ; ANERGY ; IMMUNOTHERAPY ; ERADICATION ; TOLERANCE ; MECHANISM ; REJECTION ; PREVENTS
英文摘要:

Objective To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti-tumor T cell immune responses.

Methods Examining human leucocyte antigen (HLA) and 137 expressions on 8 human blood malignancies cell lines by flow cytometry. Transfecting B7-1 gene to B7-1 negative (B7(-)) Raji and B7(-) Jurkat cell lines by liposome, and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transfection using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).

Results High level of HLA I and II molecules were expressed in most human blood malignant cell lines examined, and the co-stimulatory factor B7-2 was also highly expressed. in contrast, another member of B7 family: B7-1 was either not expressed or very limitedly expressed in most of these hematopoietic malignant cell lines. Most importantly, transfection of B7-1 gene to B7(-). Raji and B7(-). Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti-tumor T cell activation, which was demonstrated by up regulation of expression of T cell cytokines IL-2, IL-4 and INF-gamma mRNAs after incubation of these tumor cells with T cells for 24 h.

Conclusions B7 co-stimulation plays an important role in anti-tumor immunity. Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potency for activation of anti-tumor T cells. Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoietic malignancies.

语种: 英语
WOS记录号: WOS:000181239800018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54522
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Blood Dis Res, Beijing 100083, Peoples R China

Recommended Citation:
Ke, XY,Jia, LP,Wang, J,et al. Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells[J]. CHINESE MEDICAL JOURNAL,2003,116(1):78-84.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ke, XY]'s Articles
[Jia, LP]'s Articles
[Wang, J]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ke, XY]‘s Articles
[Jia, LP]‘s Articles
[Wang, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace