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学科主题: 基础医学
题名:
14-3-3 gamma affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP
作者: Li, Huihui; Guo, Yan; Teng, Junlin; Ding, Mingxiao; Yu, Albert Cheung Hoi; Chen, Jianguo
关键词: 14-3-3 gamma ; GFAP ; vimentin ; astrocytes ; phosphorylation
刊名: JOURNAL OF CELL SCIENCE
发表日期: 2006-11-01
DOI: 10.1242/jcs.03219
卷: 119, 期:21, 页:4452-4461
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: FIBRILLARY ACIDIC PROTEIN ; INTERMEDIATE-FILAMENTS ; ASTROCYTIC PROCESSES ; VIMENTIN ; KINASE ; DISEASE ; SITES ; ARCHITECTURE ; CYTOKINESIS ; PROGRESSION
英文摘要:

Recent findings indicated a protective role of GFAP in ischemic brain, injured spinal cord, and in neurodegenerative disease. We previously demonstrated that 14-3-3 gamma, once thought to be neuronal specific, was up-regulated by ischemia in astrocytes and may play a specific protective role in astrocytes. Here we report that 14-3-3 gamma associates with both soluble and filamentous GFAP in a phosphorylation- and cell-cycle- dependent manner in primary cultured astrocytes. The amount of association increases during G2/M phase due to more phosphorylated GFAP. Moreover, this interaction is independent of vimentin, another type III intermediate filament protein in astrocytes which forms glial filaments with GFAP. A series of domain deletion mutants and substitution mutations at phosphorylation sites ( from serine to alanine) on GFAP demonstrated that serine 8 in the head domain is essential for the direct association of GFAP to 14-3-3 gamma. Overexpression of 14-3-3 gamma destroyed the integrity and affected the movement of GFAP intermediate filaments. This data demonstrates that 14-3-3 gamma contributes to the regulation of dynamics of GFAP filaments, which may contribute to the stability of the cytoskeleton and the mechanisms of central nervous system neurodegenerative disease.

语种: 英语
WOS记录号: WOS:000241893200010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54529
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
2.Peking Univ, State Key Lab Biomembrane & Membrane Bioengn, Beijing 100871, Peoples R China
3.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China
4.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China
5.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
6.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Li, Huihui,Guo, Yan,Teng, Junlin,et al. 14-3-3 gamma affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP[J]. JOURNAL OF CELL SCIENCE,2006,119(21):4452-4461.
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